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An embryonic system to assess direct and indirect Wnt transcriptional targets

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Title: An embryonic system to assess direct and indirect Wnt transcriptional targets
Authors: Suresh, J
Harmston, N
Lim, KK
Kaur, P
Jin, HJ
Lusk, JB
Petretto, E
Tolwinski, NS
Item Type: Journal Article
Abstract: During animal development, complex signals determine and organize a vast number of tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pathway is involved in a variety of these cellular functions, and its signals are transmitted in part through a β-catenin/TCF transcriptional complex. Here we report an in vivo Drosophila assay that can be used to distinguish between activation, de-repression and repression of transcriptional responses, separating upstream and downstream pathway activation and canonical/non-canonical Wnt signals in embryos. We find specific sets of genes downstream of both β-catenin and TCF with an additional group of genes regulated by Wnt, while the non-canonical Wnt4 regulates a separate cohort of genes. We correlate transcriptional changes with phenotypic outcomes of cell differentiation and embryo size, showing our model can be used to characterize developmental signaling compartmentalization in vivo.
Issue Date: 11-Sep-2017
Date of Acceptance: 25-Aug-2017
URI: http://hdl.handle.net/10044/1/51968
DOI: https://dx.doi.org/10.1038/s41598-017-11519-z
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 7
Copyright Statement: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SEGMENT POLARITY GENE
PLANAR CELL POLARITY
DROSOPHILA-MELANOGASTER
SIGNALING PATHWAYS
MORPHOGEN GRADIENT
STEM-CELLS
WINGLESS
CATENIN
ARMADILLO
EXPRESSION
Publication Status: Published
Article Number: 11092
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine



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