Drift and behavior of E. coli cells

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Title: Drift and behavior of E. coli cells
Authors: Micali, G
Colin, R
Sourjik, V
Endres, RG
Item Type: Journal Article
Abstract: Chemotaxis of the bacterium Escherichia coli is well understood in shallow chemical gradients, but its swimming behavior remains difficult to interpret in steep gradients. By focusing on single-cell trajectories from simulations, we investigated the dependence of the chemotactic drift velocity on attractant concentration in an exponential gradient. Whereas maxima of the average drift velocity can be interpreted within analytical linear-response theory of chemotaxis in shallow gradients, limits in drift due to steep gradients and finite number of receptor-methylation sites for adaptation go beyond perturbation theory. For instance, we found a surprising pinning of the cells to the concentration in the gradient at which cells run out of methylation sites. To validate the positions of maximal drift, we recorded single-cell trajectories in carefully designed chemical gradients using microfluidics.
Issue Date: 27-Oct-2017
Date of Acceptance: 26-Sep-2017
URI: http://hdl.handle.net/10044/1/51639
DOI: https://dx.doi.org/10.1016/j.bpj.2017.09.031
ISSN: 0006-3495
Publisher: Biophysical Society
Start Page: 2321
End Page: 2325
Journal / Book Title: Biophysical Journal
Volume: 113
Issue: 11
Copyright Statement: © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Commission of the European Communities
Funder's Grant Number: FP7-ERC-2011-STG-280492
Keywords: Science & Technology
Life Sciences & Biomedicine
Escherichia coli
Models, Biological
Single-Cell Analysis
Escherichia coli
Models, Biological
Single-Cell Analysis
02 Physical Sciences
03 Chemical Sciences
06 Biological Sciences
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences

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