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Spontaneous charged lipid transfer between lipid vesicles

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Title: Spontaneous charged lipid transfer between lipid vesicles
Authors: Richens, JL
Tyler, AII
Barriga, HMG
Bramble, JP
Law, RV
Brooks, NJ
Seddon, JM
Ces, O
O'Shea, P
Item Type: Journal Article
Abstract: An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/ acceptor vesicle system is employed, where neutrally charged acceptor vesicles are uorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon addition of charged donor vesicles, transfer of negatively charged lipid occurs, resulting in a uorescently detectable change in the membrane potential of the acceptor vesicles. Using this approach we have studied the transfer properties of a range of lipids, varying both the headgroup and the chain length. At the low vesicle concentrations chosen, the transfer follows a rst-order process where lipid monomers are transferred presumably through the aqueous solution phase from donor to acceptor vesicle. The rate of transfer decreases with increasing chain length which is consistent with energy models previously reported for lipid monomer vesicle interactions. Our assay improves on existing methods allowing the study of a range of unmodi ed lipids, continuous monitoring of transfer and simpli ed experimental procedures.
Issue Date: 3-Oct-2017
Date of Acceptance: 7-Sep-2017
URI: http://hdl.handle.net/10044/1/51532
DOI: https://dx.doi.org/10.1038/s41598-017-12611-0
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 7
Copyright Statement: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Imperial College Trust
Funder's Grant Number: EP/J017566/1
N/A
Publication Status: Published online
Article Number: 12606
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Faculty of Natural Sciences



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