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Human CIK Cells Loaded with Au Nanorods as a Theranostic Platform for Targeted Photoacoustic Imaging and Enhanced Immunotherapy and Photothermal Therapy

Title: Human CIK Cells Loaded with Au Nanorods as a Theranostic Platform for Targeted Photoacoustic Imaging and Enhanced Immunotherapy and Photothermal Therapy
Authors: Yang, Y
Zhang, J
Xia, F
Zhang, C
Qian, Q
Zhi, X
Yue, C
Sun, R
Cheng, S
Fang, S
Jin, W
Yang, Y
Cui, D
Item Type: Journal Article
Abstract: How to realize targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy of gastric cancer has become a great challenge. Herein, we reported for the first time that human cytokine-induced killer cells (CIK) loaded with gold nanorods were used for targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy of gastric cancer. Silica-modified gold nanorods were prepared; then incubated with human cytokine-induced killer cells (CIK), resultant human CIK cells loaded with Au nanorods were evaluated for their cytotoxicity, targeted ability of gastric cancer in vitro and in vivo, immunotherapy, and photothermal therapy efficacy. In vitro cell experiment shows that human CIK cells labeled with gold nanorods actively target gastric cancer MGC803 cells, inhibit growth of MGC803 cells by inducing cell apoptosis, and kill MGC803 cells under low power density near-infrared (NIR) laser treatment (808-nm continuous wave laser, 1.5 W/cm2, 3 min). In vivo experiment results showed that human CIK cells labeled with gold nanorods could target actively and image subcutaneous gastric cancer vessels via photoacoustic imaging at 4 h post-injection, could enhance immunotherapy efficacy by up-regulating cytokines such as IL-1, IL-12, IL-2, IL-4, IL-17, and IFN-γ, and kill gastric cancer tissues by photothermal therapy via direct injection into tumor site under near-infrared (NIR) laser irradiation. High-performance human CIK cells labeled with Au nanorods are a good novel theranostic platform to exhibit great potential in applications such as tumor-targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy in the near future.
Issue Date: 6-Jun-2016
Date of Acceptance: 4-May-2016
URI: http://hdl.handle.net/10044/1/51393
DOI: https://dx.doi.org/10.1186/s11671-016-1468-8
ISSN: 1931-7573
Publisher: Springer
Journal / Book Title: Nanoscale Research Letters
Volume: 11
Copyright Statement: © 2016 Yang et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Keywords: Science & Technology
Technology
Physical Sciences
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Science & Technology - Other Topics
Materials Science
Physics
Human CIK cell
Gold nanorods
Gastric cancer cell
Immunotherapy
Photothermal therapy
Photoacoustic imaging
INDUCED KILLER-CELLS
MODIFIED GOLD NANORODS
GASTRIC-CANCER
ADOPTIVE IMMUNOTHERAPY
STEM-CELLS
T-CELLS
NANOPARTICLES
SURFACE
HYPERTHERMIA
CYTOTOXICITY
0204 Condensed Matter Physics
0912 Materials Engineering
1007 Nanotechnology
Publication Status: Published
Article Number: 285
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