Antibiotic resistance and biofilm formation among coagulase-negative staphylococci isolated from clinical samples at a tertiary care hospital of eastern Nepal

Title: Antibiotic resistance and biofilm formation among coagulase-negative staphylococci isolated from clinical samples at a tertiary care hospital of eastern Nepal
Author(s): Shrestha, LB
Bhattarai, NR
Khanal, B
Item Type: Journal Article
Abstract: Background Coagulase negative staphylococci were long regarded non-pathogenic as they are the commensals of human skin and mucosa but the recent changes in the medical practice and changes in underlying host populations, they are being considered significant pathogens associated with number of nosocomial infections. The objective of the study was to determine the species, antimicrobial susceptibility pattern, biofilm forming ability of the clinically significant CoNS isolates and to compare the different methods for the detection of biofilm formation. Methods A total of 52 clinically significant CoNS isolates obtained from different units during a year period were studied. Characterization was done using standard microbiological guidelines and antimicrobial susceptibility was done following CLSI guidelines. Biofilm formation was detected by using three methods i.e. tissue culture plate method, congo red agar method and tube adherence method. Results Among 52 isolates, S. epidermidis (52%) was the most common species which was followed by S. saprophyticus (18%) and S. haemolyticus (14%). Antimicrobial susceptibility pattern of CoNS documented resistance of 80% to ampicillin. Resistance to cefoxitin and ceftriaxone was observed in 58% of the isolates. Biofilm formation was observed in 65.38% of the isolates. The accuracy of Congo red agar and tube adherence method for the detection of biofilm formation was 82% and 76% respectively. Conclusion CoNS isolates obtained from clinical samples should be processed routinely and antimicrobial susceptibility testing should be performed. Multidrug-resistant CoNS are prevalent. All the three methods i.e. tissue culture plate, Congo red agar and tube adherence method can be used in detecting biofilm formation.
Publication Date: 31-Aug-2017
Date of Acceptance: 25-Aug-2017
URI: http://hdl.handle.net/10044/1/51171
DOI: https://dx.doi.org/10.1186/s13756-017-0251-7
ISSN: 2047-2994
Publisher: BioMed Central
Journal / Book Title: Antimicrobial Resistance and Infection Control
Volume: 6
Copyright Statement: © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Keywords: Science & Technology
Life Sciences & Biomedicine
Public, Environmental & Occupational Health
Infectious Diseases
Microbiology
CoNS
Nosocomial infection
Biofilm
SLIME PRODUCTION
SUSCEPTIBILITY
IDENTIFICATION
ADHERENCE
OXACILLIN
DEVICES
Biofilm
CoNS
Nosocomial infection
Science & Technology
Life Sciences & Biomedicine
Public, Environmental & Occupational Health
Infectious Diseases
Microbiology
CoNS
Nosocomial infection
Biofilm
SLIME PRODUCTION
SUSCEPTIBILITY
IDENTIFICATION
ADHERENCE
OXACILLIN
DEVICES
Publication Status: Published
Article Number: 89
Appears in Collections:Repository Tools



Items in Spiral are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons