Method matters: experimental evidence for shorter avian sperm in faecal compared to abdominal massage samples

File Description SizeFormat 
PONE-D-17-14246_R1accepted.pdfAccepted version1.78 MBAdobe PDFDownload
pone.0182853.pdfPublished version13.05 MBAdobe PDFDownload
Title: Method matters: experimental evidence for shorter avian sperm in faecal compared to abdominal massage samples
Author(s): Girndt, A
Cockburn, G
Sánchez-Tójar, A
Løvlie, H
Schroeder, J
Item Type: Journal Article
Abstract: Birds are model organisms in sperm biology. Previous work in zebra finches, suggested that sperm sampled from males' faeces and ejaculates do not differ in size. Here, we tested this assumption in a captive population of house sparrows, Passer domesticus. We compared sperm length in samples from three collection techniques: female dummy, faecal and abdominal massage samples. We found that sperm were significantly shorter in faecal than abdominal massage samples, which was explained by shorter heads and midpieces, but not flagella. This result might indicate that faecal sampled sperm could be less mature than sperm collected by abdominal massage. The female dummy method resulted in an insufficient number of experimental ejaculates because most males ignored it. In light of these results, we recommend using abdominal massage as a preferred method for avian sperm sampling. Where avian sperm cannot be collected by abdominal massage alone, we advise controlling for sperm sampling protocol statistically.
Publication Date: 16-Aug-2017
Date of Acceptance: 26-Jul-2017
URI: http://hdl.handle.net/10044/1/50258
DOI: https://dx.doi.org/10.1371/journal.pone.0182853
ISSN: 1932-6203
Publisher: Public Library of Science
Journal / Book Title: PLOS One
Volume: 12
Issue: 8
Copyright Statement: Copyright © 2017 Girndt et al This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Deutsche Forschungsgemeinschaft ( German Research Foundation )
Funder's Grant Number: SCHR 1447/1-1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
FINCHES TAENIOPYGIA-GUTTATA
PASSERINE BIRDS
CLOACAL PROTUBERANCE
INTRAMALE VARIATION
GALLUS-DOMESTICUS
SEXUAL SELECTION
PRACTICAL GUIDE
HOUSE SPARROW
COMPETITION
SPERMATOZOA
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Article Number: e0182853
Appears in Collections:Faculty of Natural Sciences



Items in Spiral are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons