Dynamic changes in H1 subtype composition during epigenetic reprogramming

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Title: Dynamic changes in H1 subtype composition during epigenetic reprogramming
Author(s): Hajkova, P
Schneider, R
Item Type: Journal Article
Abstract: In mammals, histone H1 consists of a family of related proteins, including five replication-dependent (H1.1–H1.5) and two replication-independent (H1.10 and H1.0) subtypes, all expressed in somatic cells. To systematically study the expression and function of H1 subtypes, we generated knockin mouse lines in which endogenous H1 subtypes are tagged. We focused on key developmental periods when epigenetic reprogramming occurs: early mouse embryos and primordial germ cell development. We found that dynamic changes in H1 subtype expression and localization are tightly linked with chromatin remodeling and might be crucial for transitions in chromatin structure during reprogramming. Although all somatic H1 subtypes are present in the blastocyst, each stage of preimplantation development is characterized by a different combination of H1 subtypes. Similarly, the relative abundance of somatic H1 subtypes can distinguish male and female chromatin upon sex differentiation in developing germ cells. Overall, our data provide new insights into the chromatin changes underlying epigenetic reprogramming. We suggest that distinct H1 subtypes may mediate the extensive chromatin remodeling occurring during epigenetic reprogramming and that they may be key players in the acquisition of cellular totipotency and the establishment of specific cellular states.
Publication Date: 9-Aug-2017
Date of Acceptance: 7-Jul-2017
URI: http://hdl.handle.net/10044/1/50078
DOI: https://dx.doi.org/10.1083/jcb.201611012
ISSN: 1540-8140
Publisher: Rockefeller University Press
Start Page: 3017
End Page: 3028
Journal / Book Title: Journal of Cell Biology
Volume: 216
Issue: 10
Copyright Statement: © 2017 Crown copyright. The government of Australia, Canada, or the UK ("the Crown") owns the copyright interests of authors who are government employees. The Crown Copyright is not transferable. This article is distributed under the terms of an Attribution–Noncommercial– Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
Sponsor/Funder: EMBO
Commission of the European Communities
Medical Research Council (MRC)
Funder's Grant Number: EMBO YIP
648879
Peter Hill MRC Doctoral Prize
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
PRIMORDIAL GERM-CELLS
MOUSE DEVELOPMENT
IN-VIVO
GENE-EXPRESSION
HISTONE
CHROMATIN
PHOSPHORYLATION
DIFFERENTIATION
PLURIPOTENCY
NUCLEOSOME
06 Biological Sciences
11 Medical And Health Sciences
Developmental Biology
Publication Status: Published
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine



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