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Chemical probes for visualizing intact animal and human brain tissue

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Cell Chemical Biology Review Manuscript_Revision 2.docxAccepted version131.44 kBMicrosoft WordView/Open
Title: Chemical probes for visualizing intact animal and human brain tissue
Authors: Lai, HM
Ng, W-L
Gentleman, SM
Wu, W
Item Type: Journal Article
Abstract: Newly developed tissue clearing techniques can be used to render intact tissues transparent. When combined with fluorescent labeling technologies and optical sectioning microscopy, this allows visualization of fine structure in three dimensions. Gene-transfection techniques have proved very useful in visualizing cellular structures in animal models, but they are not applicable to human brain tissue. Here, we discuss the characteristics of an ideal chemical fluorescent probe for use in brain and other cleared tissues, and offer a comprehensive overview of currently available chemical probes. We describe their working principles and compare their performance with the goal of simplifying probe selection for neuropathologists and stimulating probe development by chemists. We propose several approaches for the development of innovative chemical labeling methods which, when combined with tissue clearing, have the potential to revolutionize how we study the structure and function of the human brain.
Issue Date: 22-Jun-2017
Date of Acceptance: 1-Jun-2017
URI: http://hdl.handle.net/10044/1/50026
DOI: https://dx.doi.org/10.1016/j.chembiol.2017.05.015
ISSN: 2451-9456
Start Page: 659
End Page: 672
Journal / Book Title: Cell Chemical Biology
Volume: 24
Issue: 6
Copyright Statement: © 2017 Elsevier Ltd. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Parkinson's UK
Funder's Grant Number: K-1403
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
SINGLE-CELL RESOLUTION
BETA-AMYLOID PLAQUES
IN-VIVO DETECTION
MOLECULE RNA DETECTION
ALZHEIMERS-DISEASE
ALPHA-SYNUCLEIN
WHOLE-BODY
CONGO RED
CYANINE DYES
A-BETA
3D histology
brain mapping
fluorescent probes
human neuroscience
mammalian brain tissues
small-molecule probes
tissue clearing
Animals
Brain
Humans
Molecular Imaging
Molecular Probes
Publication Status: Published
Appears in Collections:Department of Medicine
Faculty of Medicine



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