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Faster, more reproducible DESI-MS for biological tissue imaging

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Title: Faster, more reproducible DESI-MS for biological tissue imaging
Authors: Tillner, J
Wu, V
Jones, EA
Pringle, SD
Karancsi, T
Dannhorn, A
Veselkov, K
McKenzie, JS
Takats, Z
Item Type: Journal Article
Abstract: A new, more robust sprayer for desorption electrospray ionization (DESI) mass spectrometry imaging is presented. The main source of variability in DESI is thought to be the uncontrolled variability of various geometric parameters of the sprayer, primarily the position of the solvent capillary, or more specifically, its positioning within the gas capillary or nozzle. If the solvent capillary is off-center, the sprayer becomes asymmetrical, making the geometry difficult to control and compromising reproducibility. If the stiffness, tip quality, and positioning of the capillary are improved, sprayer reproducibility can be improved by an order of magnitude. The quality of the improved sprayer and its potential for high spatial resolution imaging are demonstrated on human colorectal tissue samples by acquisition of images at pixel sizes of 100, 50, and 20 μm, which corresponds to a lateral resolution of 40-60 μm, similar to the best values published in the literature. The high sensitivity of the sprayer also allows combination with a fast scanning quadrupole time-of-flight mass spectrometer. This provides up to 30 times faster DESI acquisition, reducing the overall acquisition time for a 10 mm × 10 mm rat brain sample to approximately 1 h. Although some spectral information is lost with increasing analysis speed, the resulting data can still be used to classify tissue types on the basis of a previously constructed model. This is particularly interesting for clinical applications, where fast, reliable diagnosis is required. Graphical Abstract ᅟ.
Issue Date: 15-Jun-2017
Date of Acceptance: 23-Apr-2017
URI: http://hdl.handle.net/10044/1/49951
DOI: https://dx.doi.org/10.1007/s13361-017-1714-z
ISSN: 1044-0305
Publisher: Springer Verlag
Start Page: 2090
End Page: 2098
Journal / Book Title: Journal of The American Society for Mass Spectrometry
Volume: 28
Issue: 10
Copyright Statement: © The Author(s) 2017 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Sponsor/Funder: Commission of the European Communities
Funder's Grant Number: 634402
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Technology
Biochemical Research Methods
Chemistry, Analytical
Chemistry, Physical
Spectroscopy
Biochemistry & Molecular Biology
Chemistry
DESI-MS
Mass spectrometry imaging
Fast DESI
Desorption electrospray ionization
DESORPTION ELECTROSPRAY-IONIZATION
MASS-SPECTROMETRY
CANCER
CHROMATOGRAPHY
PERFORMANCE
RESOLUTION
0301 Analytical Chemistry
0306 Physical Chemistry (Incl. Structural)
0304 Medicinal And Biomolecular Chemistry
Analytical Chemistry
Publication Status: Published
Appears in Collections:Division of Surgery
Faculty of Medicine



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