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Mapping molecular function to biological nanostructure: combining structured illumination microscopy with fluorescence lifetime imaging (SIM+FLIM)

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Title: Mapping molecular function to biological nanostructure: combining structured illumination microscopy with fluorescence lifetime imaging (SIM+FLIM)
Authors: Gorlitz, F
Corcoran, DS
Garcia Castano, EA
Leitinger, B
Neil, MAA
Dunsby, CW
French, PMW
Item Type: Journal Article
Abstract: We present a new microscope integrating super-resolved imaging using structured illumination microscopy (SIM) with wide-field optically sectioned fluorescence lifetime imaging (FLIM) to provide optical mapping of molecular function and its correlation with biological nanostructure below the conventional diffraction limit. We illustrate this SIM + FLIM capability to map FRET readouts applied to the aggregation of discoidin domain receptor 1 (DDR1) in Cos 7 cells following ligand stimulation and to the compaction of DNA during the cell cycle.
Issue Date: 7-Jul-2017
Date of Acceptance: 30-Jun-2017
URI: http://hdl.handle.net/10044/1/49839
DOI: https://dx.doi.org/10.3390/photonics4030040
ISSN: 2304-6732
Publisher: MDPI
Journal / Book Title: Photonics
Volume: 4
Issue: 3
Copyright Statement: © 2017 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
Sponsor/Funder: Medical Research Council
Biotechnology and Biological Sciences Research Council
Funder's Grant Number: MR/K015834/1
BB/M006786/1
Publication Status: Published
Article Number: 40
Appears in Collections:Physics
Photonics
National Heart and Lung Institute
Faculty of Natural Sciences



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