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OPTiM: optical projection tomography integrated microscope using open-source hardware and software

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Title: OPTiM: optical projection tomography integrated microscope using open-source hardware and software
Authors: Watson
Andrews, N
Davis, S
Bugeon, L
Dallman, M
McGinty, J
Item Type: Journal Article
Abstract: We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope’s existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations’ viability.
Issue Date: 11-Jul-2017
Date of Acceptance: 21-Jun-2017
URI: http://hdl.handle.net/10044/1/49603
DOI: https://dx.doi.org/10.1371/journal.pone.0180309
ISSN: 1932-6203
Publisher: Public Library of Science
Journal / Book Title: PLOS One
Volume: 12
Issue: 7
Copyright Statement: © 2017 Watson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: BB/L018039/1
LCII_P60172
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Article Number: e0180309
Appears in Collections:Physics
Photonics
Faculty of Natural Sciences



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