NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat

Title: NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat
Authors: Serrano-Contreras, JI
Garcia-Perez, I
Melendez-Camargo, ME
Gerardo Zepeda, L
Item Type: Journal Article
Abstract: Starvation is a postabsorptive condition derived from a limitation on food resources by external factors. Energy homeostasis is maintained under this condition by using sources other than glucose via adaptive mechanisms. After refeeding, when food is available, other adaptive processes are linked to energy balance. However, less has been reported about the physiological mechanisms present as a result of these conditions, considering the rat as a supraorganism. Metabolic profiling using 1H nuclear magnetic resonance spectroscopy was used to characterize the physiological metabolic differences in urine specimens collected under starved, refed, and recovered conditions. In addition, because starvation induced lack of faecal production and not all animals produced faeces during refeeding, 24 h pooled faecal water samples were also analyzed. Urinary metabolites upregulated by starvation included 2-butanamidoacetate, 3-hydroxyisovalerate, ketoleucine, methylmalonate, p-cresyl glucuronide, p-cresyl sulfate, phenylacetylglycine, pseudouridine, creatinine, taurine, and N-acetyl glycoprotein, which were related to renal and skeletal muscle function, β-oxidation, turnover of proteins and RNA, and host–microbial interactions. Food-derived metabolites, including gut microbial cometabolites, and tricarboxylic acid cycle intermediates were upregulated under refed and recovered conditions, which characterized anabolic urinary metabotypes. The upregulation of creatine and pantothenate indicated an absorptive state after refeeding. Fecal short chain fatty acids, 3-(3-hydroxyphenyl)propionate, lactate, and acetoin provided additional information about the combinatorial metabolism between the host and gut microbiota. This investigation contributes to allow a deeper understanding of physiological responses associated with starvation and refeeding.
Issue Date: 12-Aug-2016
Date of Acceptance: 1-Aug-2016
ISSN: 1535-3893
Publisher: American Chemical Society
Start Page: 3241
End Page: 3254
Journal / Book Title: Journal of Proteome Research
Volume: 15
Issue: 9
Copyright Statement: © 2016 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, after peer review and technical editing by the publisher. To access the final edited and published work see
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
energy homeostasis
host-microbial interactions
absorptive state
host−microbial interactions
06 Biological Sciences
03 Chemical Sciences
Publication Status: Published
Appears in Collections:Division of Surgery
Department of Medicine
Faculty of Medicine

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