Enhancing continuous online microdialysis using dexamethasone: measurement of dynamic neurometabolic changes during spreading depolarization

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Title: Enhancing continuous online microdialysis using dexamethasone: measurement of dynamic neurometabolic changes during spreading depolarization
Authors: Varner, EL
Leong, CL
Jaquins-Gerstl, A
Nesbitt, KM
Boutelle, MG
Michael, AC
Item Type: Journal Article
Abstract: Microdialysis is well established in chemical neuroscience as a mainstay technology for real time intracranial chemical monitoring in both animal models and human patients. Evidence shows that microdialysis can be enhanced by mitigating the penetration injury caused during the insertion of microdialysis probes into brain tissue. Herein, we show that retrodialysis of dexamethasone in the rat cortex enhances the microdialysis detection of K(+) and glucose transients induced by spreading depolarization. Without dexamethasone, quantification of glucose transients was unreliable by 5 days after probe insertion. With dexamethasone, robust K(+) and glucose transients were readily quantified at 2 h, 5 days, and 10 days after probe insertion. The amplitudes of the K(+) transients declined day-to-day following probe insertion, and the amplitudes of the glucose transients exhibited a decreasing trend that did not reach statistical significance. Immunohistochemistry and fluorescence microscopy confirm that dexamethasone is highly effective at preserving a healthy probe-brain interface for at least 10 days even though retrodialysis of dexamethasone ceased after 5 days.
Issue Date: 8-May-2017
Date of Acceptance: 8-May-2017
URI: http://hdl.handle.net/10044/1/49128
DOI: https://dx.doi.org/10.1021/acschemneuro.7b00148
ISSN: 1948-7193
Publisher: American Chemical Society
Start Page: 1779
End Page: 1788
Journal / Book Title: ACS Chemical Neuroscience
Volume: 8
Issue: 8
Copyright Statement: © 2017 American Chemical Society.
Sponsor/Funder: Wellcome Trust
National Institutes of Health
Funder's Grant Number: 094912/Z/10/Z
0048682 - 126852-1
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Chemistry, Medicinal
Neurosciences
Pharmacology & Pharmacy
Neurosciences & Neurology
Microdialysis
dexamethasone
biosensor
microfluidic
spreading depolarization
TRAUMATIC BRAIN-INJURY
STRIATAL DOPAMINE RELEASE
HUMAN CEREBRAL-CORTEX
PENETRATION INJURY
PROBE INSERTION
RAT STRIATUM
IN-VIVO
DEPRESSION
LACTATE
GLUCOSE
Publication Status: Published
Appears in Collections:Faculty of Engineering
Bioengineering



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