From structure to mechanism – understanding initiation of DNA replication

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Title: From structure to mechanism – understanding initiation of DNA replication
Author(s): Riera, A
Barbon, M
Noguchi, Y
Reuter, ML
Schneider, S
Speck, C
Item Type: Journal Article
Abstract: DNA replication results in the doubling of the genome prior to cell division. This process requires the assembly of 50 or more protein factors into a replication fork. Here, we review recent structural and biochemical insights that start to explain how specific proteins recognize DNA replication origins, load the replicative helicase on DNA, unwind DNA, synthesize new DNA strands, and reassemble chromatin. We focus on the minichromosome maintenance (MCM2–7) proteins, which form the core of the eukaryotic replication fork, as this complex undergoes major structural rearrangements in order to engage with DNA, regulate its DNA-unwinding activity, and maintain genome stability.
Publication Date: 1-Jun-2017
Date of Acceptance: 1-Jun-2017
URI: http://hdl.handle.net/10044/1/49046
DOI: https://dx.doi.org/10.1101/gad.298232.117
ISSN: 1549-5477
Publisher: Cold Spring Harbor Laboratory Press
Start Page: 1073
End Page: 1088
Journal / Book Title: Genes & Development
Volume: 31
Copyright Statement: © 2017 Riera et al.; Published by Cold Spring Harbor Laboratory Press This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
Sponsor/Funder: Wellcome Trust
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: 107903/Z/15/Z
BB/N000323/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
Developmental Biology
Genetics & Heredity
MCM2-7
DNA replication
pre-RC
CMG
replisome
cryo-EM
ORIGIN RECOGNITION COMPLEX
DOUBLE-HEXAMERIC MCM2-7
MEIER-GORLIN SYNDROME
SINGLE-STRANDED-DNA
EUKARYOTIC REPLISOME
CMG HELICASE
BUDDING YEAST
POLYMERASE-EPSILON
ATP-HYDROLYSIS
SACCHAROMYCES-CEREVISIAE
CMG
DNA replication
MCM2–7
cryo-EM
pre-RC
replisome
Developmental Biology
06 Biological Sciences
11 Medical And Health Sciences
17 Psychology And Cognitive Sciences
Publication Status: Published
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine



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