Bovis Bacillus Calmette-Guerin (BCG) infection induces exosomal miRNA release by human macrophages

Title: Bovis Bacillus Calmette-Guerin (BCG) infection induces exosomal miRNA release by human macrophages
Authors: Alipoor, SD
Mortaz, E
Tabarsi, P
Farnia, P
Mirsaeidi, M
Garssen, J
Movassaghi, M
Adcock, IM
Item Type: Journal Article
Abstract: Background: Tuberculosis (TB) remains a significant global health concern and its diagnosis is challenging due to the limitations in the specificity and sensitivity of the current diagnostic tests. Exosomes are bioactive 30–100 nm vesicles produced by most cell types and are found in almost all human body fluids. Exosomal microRNAs (miRNAs) can transfer biological information between cells and tissues and may act as potential biomarkers in many diseases. In this pilot study, we assessed the miRNA profile of exosomes released from human monocyte-derived macrophages upon infection with Mycobacterium bovis Bacillus Calmette–Guerin (BCG). Methods: Human monocytes were obtained from the peripheral blood of three healthy subjects and driven to a monocyte-derived macrophage (MDM) phenotype using standard protocols. MDMs were infected with BCG or left uninfected as control. 72 h post-infection, exosomes were collected from the cell culture medium, RNA was isolated and RNA-seq performed. The raw reads were filtered to eliminate adaptor and primer sequences and the sequences were run against the mature human miRNA sequences available in miRBase. MicroRNAs were identified using an E value <0.01. miRNA network analysis was performed using the DIANA miRNA tool, miRDB and functional KEGG pathway analysis. Results: Infection of MDMs with BCG leads to the release of several exosomal miRNAs. These included miR-1224, -1293, -425, -4467, -4732, -484, -5094, -6848-6849, -4488 and -96 all of which were predicted to target metabolism and energy production-related pathways. Conclusions: This study provides evidence for the release of specific exosomal miRNAs from BCG-infected MDMs. These exosomal miRNAs reflect host-pathogen interaction and subversion of host metabolic processes following infection.
Issue Date: 12-May-2017
Date of Acceptance: 4-May-2017
URI: http://hdl.handle.net/10044/1/49035
DOI: https://dx.doi.org/10.1186/s12967-017-1205-9
ISSN: 1479-5876
Publisher: BIOMED CENTRAL LTD
Journal / Book Title: JOURNAL OF TRANSLATIONAL MEDICINE
Volume: 15
Issue: 1
Copyright Statement: © 2017 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/ publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated.
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 093080/Z/10/Z
Keywords: Science & Technology
Life Sciences & Biomedicine
Medicine, Research & Experimental
Research & Experimental Medicine
Mycobacterium
Exosome
miRNA
Macrophage
Biomarker
MYCOBACTERIUM-TUBERCULOSIS
LUNG-CANCER
EXTRACELLULAR VESICLES
BACTERIAL PATHOGENS
PROTEOMIC ANALYSIS
IN-VIVO
MICRORNA
BIOMARKERS
HOST
CELLS
Biomarker
Exosome
Macrophage
Mycobacterium
miRNA
Immunology
11 Medical And Health Sciences
Publication Status: Published
Article Number: ARTN 105
Appears in Collections:National Heart and Lung Institute
Airway Disease
Faculty of Medicine



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