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Cross-talk within a functional INO80 complex dimer regulates nucleosome sliding

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Title: Cross-talk within a functional INO80 complex dimer regulates nucleosome sliding
Authors: Wigley, DB
Willhoft, O
McCormack, EA
Aramayo, R
Bythell-Douglas, R
Ocloo, L
Zhang, X
Item Type: Journal Article
Abstract: Several chromatin remodellers have the ability to space nucleosomes on DNA. For ISWI remodellers, this involves an interplay between H4 histone tails, the AutoN and NegC motifs of the motor domains that together regulate ATPase activity and sense the length of DNA flanking the nucleosome. By contrast, the INO80 complex also spaces nucleosomes but is not regulated by H4 tails and lacks the AutoN and NegC motifs. Instead nucleosome sliding requires cooperativity between two INO80 complexes that monitor DNA length simultaneously on either side of the nucleosome during sliding. The C-terminal domain of the human Ino80 subunit (Ino80CTD) binds cooperatively to DNA and dimerisation of these domains provides crosstalk between complexes. ATPase activity, rather than being regulated, instead gradually becomes uncoupled as nucleosome sliding reaches an end point and this is controlled by the Ino80CTD. A single active ATPase motor within the dimer is sufficient for sliding.
Issue Date: 6-Jun-2017
Date of Acceptance: 3-Jun-2017
URI: http://hdl.handle.net/10044/1/48976
DOI: https://dx.doi.org/10.7554/eLife.25782
ISSN: 2050-084X
Publisher: eLife Sciences Publications
Journal / Book Title: eLife
Volume: 6
Copyright Statement: © 2017 Willhoft et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Sponsor/Funder: Wellcome Trust
The Royal Society
Wellcome Trust
Wellcome Trust
Cancer Research UK
Cancer Research UK
Funder's Grant Number: 091093/B/09/Z
WM110083
098412/Z/12/Z
095519/B/11/Z
12799
C6913/A21608
Keywords: Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
CHROMATIN-REMODELING COMPLEX
ACTIN-RELATED PROTEINS
STRAND BREAK REPAIR
HISTONE H4 TAIL
EXTRANUCLEOSOMAL DNA
CRYSTAL-STRUCTURE
BINDING
CHD1
ISWI
OCTAMER
Ino80
chromatin
chromosomes
cooperativity
genes
human
none
nucleosome
remodelling
Publication Status: Published
Article Number: e25782
Appears in Collections:Department of Medicine
Faculty of Medicine



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