Dual recognition element lateral flow assay (DRELFA) towards multiplex strain-specific​ influenza virus detection

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Title: Dual recognition element lateral flow assay (DRELFA) towards multiplex strain-specific​ influenza virus detection
Authors: Le, T
Chang, P
Benton, DJ
McCauley, JW
Iqbal, M
Cass, AEG
Item Type: Journal Article
Abstract: Different influenza virus strains have caused a number of recent outbreaks killing scores of people and causing significant losses in animal farming. Simple, rapid, sensitive, and specific detection of particular strains, such as a pandemic strain versus a previous seasonal influenza, plays a crucial role in the monitoring, controlling, and management of outbreaks. In this paper we describe a dual recognition element lateral flow assay (DRELFA) which pairs a nucleic acid aptamer with an antibody for use as a point-of-care platform which can detect particular strains of interest. The combination is used to overcome the individual limitations of antibodies’ cross-reactivity and aptamers’ slow binding kinetics. In the detection of influenza viruses, we show that DRELFA can discriminate a particular virus strain against others of the same subtype or common respiratory diseases while still exhibiting fast binding kinetic of the antibody-based lateral flow assay (LFA). The improvement in specificity that DRELFA exhibits is an advantage over the currently available antibody-based LFA systems for influenza viruses, which offer discrimination between influenza virus types and subtypes. Using quantitative real-time PCR (qRT-PCR), it showed that the DRELFA is very effective in localizing the analyte to the test line (consistently over 90%) and this is crucial for the sensitivity of the device. In addition, color intensities of the test lines showed a good correlation between the DRELFA and the qRT-PCR over a 50-fold concentration range. Finally, lateral flow strips with a streptavidin capture test line and an anti-antibody control line are universally applicable to specific detection of a wide range of different analytes.
Issue Date: 30-May-2017
Date of Acceptance: 30-May-2017
ISSN: 1520-6882
Publisher: American Chemical Society
Start Page: 6781
End Page: 6786
Journal / Book Title: Analytical Chemistry
Volume: 89
Issue: 12
Copyright Statement: © 2017 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Sponsor/Funder: Biotechnology and Biological Sciences Research Cou
Funder's Grant Number: PON 4090041903
Keywords: Science & Technology
Physical Sciences
Chemistry, Analytical
Analytical Chemistry
0301 Analytical Chemistry
0904 Chemical Engineering
0399 Other Chemical Sciences
Publication Status: Published
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Faculty of Natural Sciences

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