You are what you get from your fungi: nitrogen stable isotope patterns in Epipactis species

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Title: You are what you get from your fungi: nitrogen stable isotope patterns in Epipactis species
Author(s): Schiebold, JM-I
Bidartondo, MI
Karasch, P
Gravendeel, B
Gebauer, G
Item Type: Journal Article
Abstract: Background and Aims: Partially mycoheterotrophic plants are enriched in 13C and 15N compared to autotrophic plants. Here, it is hypothesized that the type of mycorrhizal fungi found in orchid roots is responsible for variation in 15N enrichment of leaf tissue in partially mycoheterotrophic orchids. Methods: The genus Epipactis was used as a case study and carbon and nitrogen isotope abundances of eight Epipactis species, fungal sporocarps of four Tuber species and autotrophic references were measured. Mycorrhizal fungi were identified using molecular methods. Stable isotope data of six additional Epipactis taxa and ectomycorrhizal and saprotrophic basidiomycetes were compiled from the literature. Key Results: The 15N enrichment of Epipactis species varied between 3·2 ± 0·8 ‰ (E. gigantea; rhizoctonia-associated) and 24·6 ± 1·6 ‰ (E. neglecta; associated with ectomycorrhizal ascomycetes). Sporocarps of ectomycorrhizal ascomycetes (10·7 ± 2·2 ‰) were significantly more enriched in 15N than ectomycorrhizal (5·2 ± 4·0 ‰) and saprotrophic basidiomycetes (3·3 ± 2·1 ‰). Conclusions: As hypothesized, it is suggested that the observed gradient in 15N enrichment of Epipactis species is strongly driven by 15N abundance of their mycorrhizal fungi; i.e. ɛ15N in Epipactis spp. associated with rhizoctonias < ɛ15N in Epipactis spp. with ectomycorrhizal basidiomycetes < ɛ15N in Epipactis spp. with ectomycorrhizal ascomycetes and basidiomycetes < ɛ15N in Epipactis spp. with ectomycorrhizal ascomycetes.
Publication Date: 22-Feb-2017
Date of Acceptance: 23-Nov-2016
URI: http://hdl.handle.net/10044/1/48737
DOI: https://dx.doi.org/10.1093/aob/mcw265
ISSN: 0305-7364
Publisher: Oxford University Press
Start Page: 1085
End Page: 1095
Journal / Book Title: ANNALS OF BOTANY
Volume: 119
Issue: 7
Copyright Statement: © 2017 The Author(s). Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com. This is a pre-copy-editing, author-produced version of an article accepted for publication in Annals of Botany following peer review. The definitive publisher-authenticated version: Julienne M.-I. Schiebold, Martin I. Bidartondo, Peter Karasch, Barbara Gravendeel, Gerhard Gebauer; You are what you get from your fungi: nitrogen stable isotope patterns in Epipactis species. Ann Bot 2017; 119 (7): 1085-1095. doi: 10.1093/aob/mcw265 is available online at: https://dx.doi.org/10.1093/aob/mcw265
Sponsor/Funder: Natural Environment Research Council (NERC)
Funder's Grant Number: NE/N009665/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Plant Sciences
Ascomycetes
basidiomycetes
carbon
Epipactis
mycorrhiza
nitrogen
Orchidaceae
partial mycoheterotrophy
stable isotopes
Tuber
ORCHID CORALLORHIZA-TRIFIDA
ECTOMYCORRHIZAL FUNGI
CARBON GAIN
PARTIAL MYCOHETEROTROPHY
NATURAL N-15
MYCORRHIZAL
PLANTS
ERICACEAE
ABUNDANCE
FOREST
Ascomycetes
Epipactis
Orchidaceae
Tuber
basidiomycetes
carbon
mycorrhiza
nitrogen
partial mycoheterotrophy
stable isotopes
Plant Biology & Botany
0607 Plant Biology
0602 Ecology
Publication Status: Published
Embargo Date: 2018-02-22
Appears in Collections:Faculty of Natural Sciences



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