Nanoscale-length control of the flagellar driveshaft requires hitting the tethered outer membrane

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Title: Nanoscale-length control of the flagellar driveshaft requires hitting the tethered outer membrane
Author(s): Cohen, EJ
Ferreira, JL
Ladinsky, MS
Beeby, M
Hughes, KT
Item Type: Journal Article
Abstract: The bacterial flagellum exemplifies a system where even small deviations from the highly regulated flagellar assembly process can abolish motility and cause negative physiological outcomes. Consequently, bacteria have evolved elegant and robust regulatory mechanisms to ensure that flagellar morphogenesis follows a defined path, with each component self-assembling to predetermined dimensions. The flagellar rod acts as a driveshaft to transmit torque from the cytoplasmic rotor to the external filament. The rod self-assembles to a defined length of ~25 nanometers. Here, we provide evidence that rod length is limited by the width of the periplasmic space between the inner and outer membranes. The length of Braun's lipoprotein determines periplasmic width by tethering the outer membrane to the peptidoglycan layer.
Publication Date: 14-Apr-2017
Date of Acceptance: 17-Mar-2017
URI: http://hdl.handle.net/10044/1/48407
DOI: https://dx.doi.org/10.1126/science.aam6512
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science
Start Page: 197
End Page: 200
Journal / Book Title: SCIENCE
Volume: 356
Issue: 6334
Copyright Statement: © 2017 American Association for the Advancement of Science. This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science 14 Apr 2017: Vol. 356, Issue 6334, pp. 197-200 DOI: 10.1126/science.aam6512
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
ENTERICA SEROVAR TYPHIMURIUM
SALMONELLA-TYPHIMURIUM
ESCHERICHIA-COLI
BASAL-BODY
L-RING
OSMOTIC-STRESS
HOOK LENGTH
LIPOPROTEIN
PROTEIN
FLGG
General Science & Technology
MD Multidisciplinary
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences



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