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Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein

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Title: Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein
Authors: Tabib-Salazar, A
Liu, B
Shadrin, A
Burchell, L
Wang, Z
Wang, Z
Goren, MG
Yosef, I
Qimron, U
Severinov, K
Matthews, SJ
Wigneshweraraj, S
Item Type: Journal Article
Abstract: Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the bacterial RNA polymerase (RNAP) by the 7 kDa T7 protein Gp2. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.7, which adopts a winged helix-turn-helix-like structure and specifically represses transcription initiation from host RNAP-dependent promoters on the phage genome via a mechanism that involves interaction with DNA and the bacterial RNAP. Whereas Gp2 is indispensable for T7 growth in E. coli, we show that Gp5.7 is required for optimal infection outcome. Our findings provide novel insights into how phages fine-tune the activity of the host transcription machinery to ensure both successful and efficient phage progeny development.
Issue Date: 9-May-2017
Date of Acceptance: 21-Apr-2017
URI: http://hdl.handle.net/10044/1/48259
DOI: https://dx.doi.org/10.1093/nar/gkx370
ISSN: 1362-4962
Publisher: Oxford University Press (OUP)
Start Page: 7697
End Page: 7707
Journal / Book Title: Nucleic Acids Research
Volume: 45
Issue: 13
Copyright Statement: © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/ 4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Wellcome Trust
Wellcome Trust
Wellcome Trust
Funder's Grant Number: BB/K000233/1
100958/Z/13/Z
100280/Z/12/Z
WT/104933/z/14/z
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
BACTERIOPHAGE T7
H-NS
TRANSCRIPTION
SUBUNIT
GENE
REPLICATION
INHIBITION
MECHANISM
GROWTH
HOSTS
05 Environmental Sciences
06 Biological Sciences
08 Information And Computing Sciences
Developmental Biology
Publication Status: Published
Appears in Collections:Department of Medicine
Faculty of Medicine
Faculty of Natural Sciences



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