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Peptide-functionalized fluorescent particles for in situ detection of nitric oxide via peroxynitrite-mediated nitration

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Title: Peptide-functionalized fluorescent particles for in situ detection of nitric oxide via peroxynitrite-mediated nitration
Authors: Chang, JYH
Chow, LW
Dismuke, WM
Ethier, CR
Stevens, MM
Stamer, WD
Overby, D
Item Type: Journal Article
Abstract: Nitric oxide (NO) is a free radical signaling molecule that plays a crucial role in modulating physiological homeostasis across multiple biological systems. NO dysregulation is linked to the pathogenesis of multiple diseases; therefore, its quantification is important for understanding pathophysiological processes. The detection of NO is challenging, typically limited by its reactive nature and short half-life. Additionally, the presence of interfering analytes and accessibility to biological fluids in the native tissues make the measurement technically challenging and often unreliable. Here, a bio-inspired peptide-based NO sensor is developed, which detects NO-derived oxidants, predominately peroxynitrite-mediated nitration of tyrosine residues. It is demonstrated that these peptide-based NO sensors can detect peroxynitrite-mediated nitration in response to physiological shear stress by endothelial cells in vitro. Using the peptide-conjugated fluorescent particle immunoassay, peroxynitrite-mediated nitration activity with a detection limit of ≈100 × 10−9m is detected. This study envisions that the NO detection platform can be applied to a multitude of applications including monitoring of NO activity in healthy and diseased tissues, localized detection of NO production of specific cells, and cell-based/therapeutic screening of peroxynitrite levels to monitor pronitroxidative stress in biological samples.
Issue Date: 17-May-2017
Date of Acceptance: 12-Apr-2017
URI: http://hdl.handle.net/10044/1/48112
DOI: https://dx.doi.org/10.1002/adhm.201700383
ISSN: 2192-2640
Publisher: Wiley
Journal / Book Title: Advanced Healthcare Materials
Volume: 6
Issue: 16
Copyright Statement: © 2017 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Wellcome Trust
Commission of the European Communities
Funder's Grant Number: 088844/Z/09/Z
ERC-2013-CoG-616417
Keywords: Science & Technology
Technology
Engineering, Biomedical
Nanoscience & Nanotechnology
Materials Science, Biomaterials
Engineering
Science & Technology - Other Topics
Materials Science
endothelial cells
immunoassays
nitric oxide detection
peptide biosensors
peroxynitrite
PROTEIN-TYROSINE NITRATION
TUMOR MICROVASCULAR PERMEABILITY
SOLUBLE GUANYLATE-CYCLASE
SHEAR-STRESS
MASS-SPECTROMETRY
OXIDATIVE STRESS
PROSTACYCLIN SYNTHASE
BIOLOGICAL-SYSTEMS
SUPEROXIDE
RELEASE
Publication Status: Published
Article Number: 1700383
Appears in Collections:Faculty of Engineering
Materials
Bioengineering
Faculty of Natural Sciences



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