Flow dynamics and HSPC homing in bone marrow microvessels.

File Description SizeFormat 
1-s2.0-S2211124717300979-main.pdfPublished version5.31 MBAdobe PDFView/Open
Title: Flow dynamics and HSPC homing in bone marrow microvessels.
Authors: Bixel, MG
Kusumbe, AP
Ramasamy, SK
Sivaraj, KK
Butz, S
Vestweber, D
Adams, RH
Item Type: Journal Article
Abstract: Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters. In addition, we observed the homing of individual hematopoietic stem and progenitor cells and obtained detailed information on their homing behavior. With our imaging setup, we determined flow patterns at cellular resolution, blood flow velocities and wall shear stress in small arterial vessels and highly branched sinusoidal capillaries, and the cellular dynamics of hematopoietic stem and progenitor cell homing.
Issue Date: 14-Feb-2017
Date of Acceptance: 18-Jan-2017
ISSN: 2211-1247
Publisher: Elsevier
Start Page: 1804
End Page: 1816
Journal / Book Title: Cell Reports
Volume: 18
Issue: 7
Copyright Statement: © 2017 The Author(s). This is an open access article under the CC BY-NC-ND license (
Keywords: blood flow velocities
bone marrow
deep tissue imaging
hematopoietic parameters
hematopoietic stem cell
intravital imaging
stem cell homing
wall shear stress
Publication Status: Published
Conference Place: United States
Open Access location:
Appears in Collections:Clinical Sciences
Molecular Sciences

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commonsx