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Elevated rate of genome rearrangements in radiation-resistant bacteria

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Title: Elevated rate of genome rearrangements in radiation-resistant bacteria
Authors: Repar, J
Supek, F
Klanjscek, T
Warnecke, T
Zahradka, K
Zahradka, D
Item Type: Journal Article
Abstract: A number of bacterial, archaeal, and eukaryotic species are known for their resistance to ionizing radiation. One of the challenges these species face is a potent environmental source of DNA double-strand breaks, potential drivers of genome structure evolution. Efficient and accurate DNA double-strand break repair systems have been demonstrated in several unrelated radiation-resistant species and are putative adaptations to the DNA damaging environment. Such adaptations are expected to compensate for the genome-destabilizing effect of environmental DNA damage and may be expected to result in a more conserved gene order in radiation-resistant species. However, here we show that rates of genome rearrangements, measured as loss of gene order conservation with time, are higher in radiation-resistant species in multiple, phylogenetically independent groups of bacteria. Comparison of indicators of selection for genome organization between radiation-resistant and phylogenetically matched, non-resistant species argues against tolerance to disruption of genome structure as a strategy for radiation resistance. Interestingly, an important mechanism affecting genome rearrangements in prokaryotes, the symmetrical inversions around origin of DNA replication, shapes genome structure of both radiation-resistant and non-resistant species. In conclusion, the opposing effects of environmental DNA damage and DNA repair result in elevated rates of genome rearrangements in radiation-resistant bacteria.
Issue Date: 30-Mar-2017
Date of Acceptance: 30-Jan-2017
URI: http://hdl.handle.net/10044/1/45028
DOI: https://dx.doi.org/10.1534/genetics.116.196154
ISSN: 1943-2631
Publisher: Genetics Society of America
Start Page: 1677
End Page: 1689
Journal / Book Title: Genetics
Volume: 205
Issue: 4
Copyright Statement: © 2017 Repar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Imperial College London
Medical Research Council
Funder's Grant Number: Junior Research Fellowship
MC_A658_5TY40
Keywords: Deinococcus radiodurans
gamma radiation
gene order
genome stability
synteny
Developmental Biology
0604 Genetics
Publication Status: Published
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine



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