Elevated rate of genome rearrangements in radiation-resistant bacteria

File Description SizeFormat 
1677.full.pdfPublished version1.43 MBAdobe PDFView/Open
Title: Elevated rate of genome rearrangements in radiation-resistant bacteria
Authors: Repar, J
Supek, F
Klanjscek, T
Warnecke, T
Zahradka, K
Zahradka, D
Item Type: Journal Article
Abstract: A number of bacterial, archaeal, and eukaryotic species are known for their resistance to ionizing radiation. One of the challenges these species face is a potent environmental source of DNA double-strand breaks, potential drivers of genome structure evolution. Efficient and accurate DNA double-strand break repair systems have been demonstrated in several unrelated radiation-resistant species and are putative adaptations to the DNA damaging environment. Such adaptations are expected to compensate for the genome-destabilizing effect of environmental DNA damage and may be expected to result in a more conserved gene order in radiation-resistant species. However, here we show that rates of genome rearrangements, measured as loss of gene order conservation with time, are higher in radiation-resistant species in multiple, phylogenetically independent groups of bacteria. Comparison of indicators of selection for genome organization between radiation-resistant and phylogenetically matched, non-resistant species argues against tolerance to disruption of genome structure as a strategy for radiation resistance. Interestingly, an important mechanism affecting genome rearrangements in prokaryotes, the symmetrical inversions around origin of DNA replication, shapes genome structure of both radiation-resistant and non-resistant species. In conclusion, the opposing effects of environmental DNA damage and DNA repair result in elevated rates of genome rearrangements in radiation-resistant bacteria.
Issue Date: 30-Mar-2017
Date of Acceptance: 30-Jan-2017
ISSN: 1943-2631
Publisher: Genetics Society of America
Start Page: 1677
End Page: 1689
Journal / Book Title: Genetics
Volume: 205
Issue: 4
Copyright Statement: © 2017 Repar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Imperial College London
Medical Research Council
Funder's Grant Number: Junior Research Fellowship
Keywords: Deinococcus radiodurans
gamma radiation
gene order
genome stability
Developmental Biology
0604 Genetics
Publication Status: Published
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons