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Inactivation, clearance, and functional effects of lung-instilled short and long silver nanowires in rats

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Title: Inactivation, clearance, and functional effects of lung-instilled short and long silver nanowires in rats
Authors: Chung, KF
Seiffert, J
Chen, S
Theodorou, IG
Goode, AE
Leo, BF
McGilvery, CM
Hussain, F
Wiegman, C
Rossios, C
Zhu, J
Gong, J
Tariq, F
Yufit, V
Monteith, AJ
Hashimoto, T
Skepper, JN
Ryan, MP
Zhang, J
Tetley, TD
Porter, AE
Item Type: Journal Article
Abstract: There is a potential for silver nanowires (AgNWs) to be inhaled, but there is little information on their health effects and their chemical transformation inside the lungs in vivo. We studied the effects of short (S-AgNWs; 1.5 μm) and long (L-AgNWs; 10 μm) nanowires instilled into the lungs of Sprague–Dawley rats. S- and L-AgNWs were phagocytosed and degraded by macrophages; there was no frustrated phagocytosis. Interestingly, both AgNWs were internalized in alveolar epithelial cells, with precipitation of Ag2S on their surface as secondary Ag2S nanoparticles. Quantitative serial block face three-dimensional scanning electron microscopy showed a small, but significant, reduction of NW lengths inside alveolar epithelial cells. AgNWs were also present in the lung subpleural space where L-AgNWs exposure resulted in more Ag+ve macrophages situated within the pleura and subpleural alveoli, compared with the S-AgNWs exposure. For both AgNWs, there was lung inflammation at day 1, disappearing by day 21, but in bronchoalveolar lavage fluid (BALF), L-AgNWs caused a delayed neutrophilic and macrophagic inflammation, while S-AgNWs caused only acute transient neutrophilia. Surfactant protein D (SP-D) levels in BALF increased after S- and L-AgNWs exposure at day 7. L-AgNWs induced MIP-1α and S-AgNWs induced IL-18 at day 1. Large airway bronchial responsiveness to acetylcholine increased following L-AgNWs, but not S-AgNWs, exposure. The attenuated response to AgNW instillation may be due to silver inactivation after precipitation of Ag2S with limited dissolution. Our findings have important consequences for the safety of silver-based technologies to human health.
Issue Date: 21-Feb-2017
Date of Acceptance: 21-Feb-2017
URI: http://hdl.handle.net/10044/1/44941
DOI: https://dx.doi.org/10.1021/acsnano.6b07313
ISSN: 1936-086X
Publisher: American Chemical Society
Start Page: 2652
End Page: 2664
Journal / Book Title: ACS Nano
Volume: 11
Issue: 3
Copyright Statement: © 2017 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
Sponsor/Funder: Natural Environment Research Council (NERC)
National Institutes of Health
Funder's Grant Number: NE/H012893/1
H50669
Keywords: alveolar epithelial cells
bronchial hyperresponsiveness
macrophages
silver nanowires
silver sulfidation
surfactant protein D
Nanoscience & Nanotechnology
MD Multidisciplinary
Publication Status: Published
Appears in Collections:Materials
National Heart and Lung Institute
Airway Disease
Faculty of Medicine
Faculty of Natural Sciences



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