A new apparatus to induce lysis of planktonic microbial cells by shock compression, cavitation and spray

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Title: A new apparatus to induce lysis of planktonic microbial cells by shock compression, cavitation and spray
Author(s): Schiffer, A
Gardner, M
Lynn, R
Tagarielli, V
Item Type: Journal Article
Abstract: E xperiments were conducted on an aqueous growth medium containing cultures of Escherichia c oli ( E. coli ) XL1 - Blue , to investigate , in a single experiment, the effect of two types of dynamic mechanical loading on cellular integrity . A bespoke shock tube was used to subject separate portions of a planktonic bacteria l culture to two different loading sequences: (i) shock compression followed by cavitation, and (ii ) shock compression followed by spray . T he apparatus allow s the generati on of a n adjustable lo a ding shock wave of magnitude up to 3 00 M Pa in a sterile laboratory environment. C ultures of E. coli were tested with this apparatus and the s pread - plate technique was used to measure the survivability after mechanical loading . T he loading sequence (ii) gave higher mortality than (i), suggesting that the bacteria are more vulnerable to shear deformation and cavitation than to hydrostatic compressi on . We present results of preliminary experi ments and suggestions for further experimental work; we discuss the potential applications of this technique to sterilise large volumes of fluid samples.
Publication Date: 15-Mar-2017
Date of Acceptance: 14-Feb-2017
URI: http://hdl.handle.net/10044/1/44678
DOI: https://dx.doi.org/10.1098/rsos.160939
ISSN: 2054-5703
Publisher: Royal Society, The
Journal / Book Title: Royal Society Open Science
Volume: 4
Copyright Statement: © 2017 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Sponsor/Funder: Engineering & Physical Science Research Council (E
Funder's Grant Number: AESZ_P44345
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
shock wave
Escherichia coli
cell lysis
Escherichia coli
cell lysis
shock wave
Publication Status: Published
Article Number: 160939
Appears in Collections:Faculty of Engineering

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