Altmetric

DNA methylome analysis identifies accelerated epigenetic aging associated with postmenopausal breast cancer susceptibility

File Description SizeFormat 
170123EJC-D-16-01658R1_MS.pdfAccepted version1.42 MBAdobe PDFView/Open
Title: DNA methylome analysis identifies accelerated epigenetic aging associated with postmenopausal breast cancer susceptibility
Authors: Ambatipudi, S
Horvath, S
Perrier, F
Cuenin, C
Hernandez-Vargas, H
Le Calvez-Kelm, F
Durand, G
Byrnes, G
Ferrari, P
Bouaoun, L
Sklias, A
Chajes, V
Overvad, K
Severi, G
Baglietto, L
Clavel-Chapelon, F
Kaaks, R
Barrdah, M
Boeing, H
Trichopoulou, A
Lagiou, P
Naska, A
Masala, G
Agnoli, C
Polidoro, S
Tumino, R
Panico, S
Dollé, M
Peeters, PHM
Onland-Moret, NC
Sandanger, TM
Nøst, TH
Weiderpass Vainio, E
Ramón Quirós, J
Agudo, A
Rodriguez-Barranco, M
Huerta Castaño, JM
Barricarte, A
Matheu Fernández, A
Travis, RC
Vineis, P
Muller, DC
Riboli, E
Gunter, M
Romieu, I
Herceg, Z
Item Type: Journal Article
Abstract: Aim of the study A vast majority of human malignancies are associated with ageing, and age is a strong predictor of cancer risk. Recently, DNA methylation-based marker of ageing, known as ‘epigenetic clock’, has been linked with cancer risk factors. This study aimed to evaluate whether the epigenetic clock is associated with breast cancer risk susceptibility and to identify potential epigenetics-based biomarkers for risk stratification. Methods Here, we profiled DNA methylation changes in a nested case–control study embedded in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort (n = 960) using the Illumina HumanMethylation 450K BeadChip arrays and used the Horvath age estimation method to calculate epigenetic age for these samples. Intrinsic epigenetic age acceleration (IEAA) was estimated as the residuals by regressing epigenetic age on chronological age. Results We observed an association between IEAA and breast cancer risk (OR, 1.04; 95% CI, 1.007–1.076, P = 0.016). One unit increase in IEAA was associated with a 4% increased odds of developing breast cancer (OR, 1.04; 95% CI, 1.007–1.076). Stratified analysis based on menopausal status revealed that IEAA was associated with development of postmenopausal breast cancers (OR, 1.07; 95% CI, 1.020–1.11, P = 0.003). In addition, methylome-wide analyses revealed that a higher mean DNA methylation at cytosine-phosphate-guanine (CpG) islands was associated with increased risk of breast cancer development (OR per 1 SD = 1.20; 95 %CI: 1.03–1.40, P = 0.02) whereas mean methylation levels at non-island CpGs were indistinguishable between cancer cases and controls. Conclusion Epigenetic age acceleration and CpG island methylation have a weak, but statistically significant, association with breast cancer susceptibility.
Issue Date: 28-Feb-2017
Date of Acceptance: 20-Jan-2017
URI: http://hdl.handle.net/10044/1/44191
DOI: https://dx.doi.org/10.1016/j.ejca.2017.01.014
ISSN: 0014-2964
Publisher: Elseiver
Start Page: 299
End Page: 307
Journal / Book Title: European Journal of Cancer
Volume: 75
Copyright Statement: © 2017 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: University Medical Center Utrecht
Imperial College Trust
Funder's Grant Number: N/A
P47328
Keywords: Age acceleration
Biomarkers
Breast cancer
DNA methylation
Epigenomics
Prospective studies
Oncology & Carcinogenesis
1112 Oncology And Carcinogenesis
Publication Status: Published
Appears in Collections:Faculty of Medicine
Epidemiology, Public Health and Primary Care



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commonsx