Biocompatibility and bioactivity of porous polymer-derived Ca-Mg silicate ceramics.

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Title: Biocompatibility and bioactivity of porous polymer-derived Ca-Mg silicate ceramics.
Author(s): Fiocco, L
Li, S
Stevens, MM
Bernardo, E
Jones, JR
Item Type: Journal Article
Abstract: Magnesium is a trace element in the human body, known to have important effects on cell differentiation and the mineralisation of calcified tissues. This study aimed to synthesise highly porous Ca-Mg silicate foamed scaffolds from preceramic polymers, with analysis of their biological response. Akermanite (Ak) and wollastonite-diopside (WD) ceramic foams were obtained from the pyrolysis of a liquid silicone mixed with reactive fillers. The porous structure was obtained by controlled water release from selected fillers (magnesium hydroxide and borax) at 350°C. The homogeneous distribution of open pores, with interconnects of modal diameters of 160-180μm was obtained and maintained after firing at 1100°C. Foams, with porosity exceeding 80%, exhibited compressive strength values of 1-2MPa. In vitro studies were conducted by immersion in SBF for 21days, showing suitable dissolution rates, pH and ionic concentrations. Cytotoxicity analysis performed in accordance with ISO10993-5 and ISO10993-12 standards confirmed excellent biocompatibility of both Ak and WD foams. In addition, MC3T3-E1 cells cultured on the Mg-containing scaffolds demonstrated enhanced osteogenic differentiation and the expression of osteogenic markers including Collagen Type I, Osteopontin and Osteocalcin, in comparison to Mg-free counterparts. The results suggest that the addition of magnesium can further enhance the bioactivity and the potential for bone regeneration applications of Ca-silicate materials. STATEMENTS OF SIGNIFICANCE: Here, we show that the incorporation of Mg in Ca-silicates plays a significant role in the enhancement of the osteogenic differentiation and matrix formation of MC3T3-E1 cells, cultured on polymer-derived highly porous scaffolds. Reduced degradation rates and improved mechanical properties are also observed, compared to Mg-free counterparts, suggesting the great potential of Ca-Mg silicates as bone tissue engineering materials. Excellent biocompatibility of the new materials, in accordance to the ISO10993-5 and ISO10993-12 standard guidelines, confirms the preceramic polymer route as an efficient synthesis methodology for bone scaffolds. The use of hydrated fillers as porogens is an additional novelty feature presented in the manuscript.
Publication Date: 23-Dec-2016
Date of Acceptance: 21-Dec-2016
URI: http://hdl.handle.net/10044/1/43928
DOI: https://dx.doi.org/10.1016/j.actbio.2016.12.043
ISSN: 1878-7568
Publisher: Elsevier
Start Page: 56
End Page: 67
Journal / Book Title: Acta Biomaterialia
Volume: 50
Copyright Statement: © 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords: Science & Technology
Technology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
Ca-Mg silicates
Bioactive
Preceramic polymers
Porous
Ceramic foams
BONE-MINERAL DENSITY
IN-VITRO
IONIC PRODUCTS
OSTEOBLASTIC DIFFERENTIATION
OSTEOGENIC SUPPLEMENTS
BIOLOGICAL-PROPERTIES
FETAL OSTEOBLASTS
DIETARY MAGNESIUM
DISRUPTS BONE
METABOLISM
Bioactive
Ca-Mg silicates
Ceramic foams
Porous
Preceramic polymers
Biomedical Engineering
MD Multidisciplinary
Publication Status: Published
Conference Place: England
Embargo Date: 2017-12-23
Appears in Collections:Faculty of Engineering
Materials
Faculty of Natural Sciences



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