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Medical swab analysis using desorption electrospray ionization mass spectrometry (DESI-MS) – a non-invasive approach for mucosal diagnostics

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Title: Medical swab analysis using desorption electrospray ionization mass spectrometry (DESI-MS) – a non-invasive approach for mucosal diagnostics
Authors: Pruski, P
MacIntyre, DA
Lewis, HV
Inglese, P
Dos Santos Correia, G
Hansel, TT
Bennett, PR
Holmes, E
Takats, Z
Item Type: Journal Article
Abstract: Medical swabs are routinely used worldwide to sample human mucosa for microbiological screening with culture methods. These are usually time-consuming and have a narrow focus on screening for particular microorganism species. As an alternative, direct mass spectrometric profiling of the mucosal metabolome provides a broader window into the mucosal ecosystem. We present for the first time a minimal effort/minimal-disruption technique for augmenting the information obtained from clinical swab analysis with mucosal metabolome profiling using desorption electrospray ionization mass spectrometry (DESI-MS) analysis. Ionization of mucosal biomass occurs directly from a standard rayon swab mounted on a rotating device and analyzed by DESI MS using an optimized protocol considering swab–inlet geometry, tip–sample angles and distances, rotation speeds, and reproducibility. Multivariate modeling of mass spectral fingerprints obtained in this way readily discriminate between different mucosal surfaces and display the ability to characterize biochemical alterations induced by pregnancy and bacterial vaginosis (BV). The method was also applied directly to bacterial biomass to confirm the ability to detect intact bacterial species from a swab. These results highlight the potential of direct swab analysis by DESI-MS for a wide range of clinical applications including rapid mucosal diagnostics for microbiology, immune responses, and biochemistry.
Issue Date: 27-Dec-2016
Date of Acceptance: 27-Dec-2016
URI: http://hdl.handle.net/10044/1/43887
DOI: https://dx.doi.org/10.1021/acs.analchem.6b03405
ISSN: 0003-2700
Publisher: American Chemical Society
Start Page: 1540
End Page: 1550
Journal / Book Title: Analytical Chemistry
Volume: 89
Issue: 3
Copyright Statement: © 2016 American Chemical Society. This is the author's version of the work. It is posted here by permission of ACM for your personal use. Not for redistribution. The definitive version was published in Analytical Chemistry, December 27, 2016, https://dx.doi.org/10.1021/acs.analchem.6b03405
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Commission of the European Communities
Imperial College Healthcare NHS Trust- BRC Funding
Funder's Grant Number: MR/L009226/1
MR/L009226/1
617896
RDD03
Keywords: Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
CLINICAL MICROBIOLOGY
IN-VITRO
BACTERIA
IDENTIFICATION
FLUID
INFECTION
DIFFERENTIATION
MECHANISMS
IMMUNITY
DEFENSE
Analytical Chemistry
0301 Analytical Chemistry
0904 Chemical Engineering
0399 Other Chemical Sciences
Publication Status: Published
Appears in Collections:Division of Surgery
National Heart and Lung Institute
Faculty of Medicine



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