Rapid Evaporative Ionisation Mass Spectrometry (REIMS) provides accurate direct from culture species identification within the genus Candida

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Title: Rapid Evaporative Ionisation Mass Spectrometry (REIMS) provides accurate direct from culture species identification within the genus Candida
Authors: Cameron, SJS
Bolt, F
Perdones-Montero, A
Rickards, T
Hardiman, K
Abdolrasouli, A
Burke, A
Bodai, Z
Karancsi, T
Simon, D
Schaffer, R
Rebec, M
Balog, J
Takats, Z
Item Type: Journal Article
Abstract: Members of the genus Candida, such as C. albicans and C. parapsilosis, are important human pathogens. Other members of this genus, previously believed to carry minimal disease risk, are increasingly recognised as important human pathogens, particularly because of variations in susceptibilities to widely used anti-fungal agents. Thus, rapid and accurate identification of clinical Candida isolates is fundamental in ensuring timely and effective treatments are delivered. Rapid Evaporative Ionisation Mass Spectrometry (REIMS) has previously been shown to provide a high-throughput platform for the rapid and accurate identification of bacterial and fungal isolates. In comparison to commercially available matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI-ToF), REIMS based methods require no preparative steps nor time-consuming cell extractions. Here, we report on the ability of REIMS-based analysis to rapidly and accurately identify 153 clinical Candida isolates to species level. Both handheld bipolar REIMS and high-throughput REIMS platforms showed high levels of species classification accuracy, with 96% and 100% of isolates classified correctly to species level respectively. In addition, significantly different (FDR corrected P value < 0.05) lipids within the 600 to 1000 m/z mass range were identified, which could act as species-specific biomarkers in complex microbial communities.
Issue Date: 14-Nov-2016
Date of Acceptance: 13-Oct-2016
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 6
Copyright Statement: © 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Micromass UK Ltd
Funder's Grant Number: BB/L020858/1
ICL 01/02/03
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Publication Status: Published
Article Number: ARTN 36788
Appears in Collections:Division of Surgery

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