Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging

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Title: Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging
Authors: Sparks, H
Gorlitz, F
Kelly, D
Warren, SC
Kellet, PA
Garcia, E
Dymoke-Bradshaw, AKL
Hares, JD
Neil, MAA
Dunsby, C
French, PMW
Item Type: Journal Article
Abstract: We report the characterisation of gated optical image intensifiers for fluorescence lifetime imaging (FLIM), evaluating the performance of several different prototypes that culminate in a new design that provides improved spatial resolution conferred b y the addition of a magnetic field to reduce the lateral spread of photoelectrons on their path between the photocathode and microchannel plate, and higher signal to noise ratio conferred by longer time gates. We also present a methodology to compare these systems and their capabilities, including the quantitative readouts of F ö rster resonant energy transfer (FRET).
Issue Date: 26-Jan-2017
Date of Acceptance: 26-Dec-2016
URI: http://hdl.handle.net/10044/1/43748
DOI: https://dx.doi.org/10.1063/1.4973917
ISSN: 1089-7623
Publisher: AIP Publishing
Journal / Book Title: Review of Scientific Instruments
Volume: 88
Copyright Statement: © 2017 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council
Funder's Grant Number: EP/I02770X/1
BB/M006786/1
BB/M006786/1
Keywords: Science & Technology
Technology
Physical Sciences
Instruments & Instrumentation
Physics, Applied
Physics
PLATE READER
TIME-DOMAIN
MICROSCOPY
SPEED
FLIM
FRET
EXCITATION
RESOLUTION
SYSTEM
LIGHT
Applied Physics
09 Engineering
02 Physical Sciences
03 Chemical Sciences
Publication Status: Published
Article Number: 013707
Appears in Collections:Physics
Photonics
Faculty of Natural Sciences



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