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Basophils, high-affinity IgE receptors and CCL2 in human anaphylaxis

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Title: Basophils, high-affinity IgE receptors and CCL2 in human anaphylaxis
Authors: Turner, PJ
Custovic, A
Shamji, MH
Item Type: Journal Article
Abstract: Background : The role of basophils in anaphylaxis is un clear . Objective : T o investigate whether basophils have an important role in human anaphylaxis . Methods : In an emergency department study, w e recruited 31 patients with acute anaphylaxis , predominantly to hymenoptera venom. We measured expression of basophil activation markers ( CD63 , CD203c) , the absolute number of circulating basophils, whole - blood FcεRI , CPA3 and HDC gene expression , and serum markers ( CCL2 , CCL5, CCL11, IL - 3 , TSLP ) at three time points ( during the anaphylactic episode , and in convalescent samples 7 and 30 day s later ). We recruited 134 h ymenoptera - allergic and 76 healthy controls for comparison . We then investigated whether the changes observed during venom - related anaphylaxis also occur during allergic reactions to food in 22 peanut - allergic individuals undergoing double - blind placebo - controlled food challenge to peanut (DBPC F C). Results : The number of circulating basophils was significantly lower during anaphylaxis (median 3.5 cells/μl) than 7 and 30 days later (17.5 and 24.7 cells/μl , P<0.0001 ) , and compared to venom - allerg ic and healthy controls (21 and 23.4 cells/μl , P <0.0001 ) . FcεRI expression during anaphylaxis w as also significantly lower than in convalescent samples ( P≤ 0.00 2) and venom - allerg ic control s ( P <0.0001 ). CCL2 (but not other serum markers) was significant ly higher during anaphylaxis (median 658 pg/ml) than in convalescent samples (314 and 311 pg/ml , 7 and 30 days , P<0.001). Peanut - induced allergic reactions resulted in a significant decrease in circulating basop hils compared to pre - challenge sample s (P=0.016), a decrease in FcεRI expression (P=0.007) , and a n increase in CCL2 (P=0.003) . Conclusions : O ur findings imply an important and specific role for basophils in the pathophysiology of human anaphylaxis.
Issue Date: 22-Mar-2017
Date of Acceptance: 23-Dec-2016
URI: http://hdl.handle.net/10044/1/43746
DOI: https://dx.doi.org/10.1016/j.jaci.2016.12.989
ISSN: 1097-6825
Publisher: Elsevier
Start Page: 750
End Page: 758.e15
Journal / Book Title: Journal of Allergy and Clinical Immunology
Volume: 140
Issue: 3
Copyright Statement: 2017 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Medical Research Council (MRC)
National Institute for Health Research
Funder's Grant Number: MR/K010468/1
RDD02
Keywords: Science & Technology
Life Sciences & Biomedicine
Allergy
Immunology
Anaphylaxis
basophils
CD63 activation
Fc epsilon RI expression
CCL2
serum tryptase
PLATELET-ACTIVATING-FACTOR
INSECT-STING CHALLENGE
ALLERGIC REACTIONS
MAST-CELL
ATOPIC-DERMATITIS
FOOD CHALLENGES
TRYPTASE LEVELS
TRANSENDOTHELIAL MIGRATION
VENOM IMMUNOTHERAPY
IMMUNOGLOBULIN-E
FcεRI expression
Adolescent
Adult
Aged
Animals
Basophils
Cytokines
Double-Blind Method
Female
Gene Expression
Humans
Hymenoptera
Leukocyte Count
Male
Middle Aged
Peanut Hypersensitivity
Receptors, IgE
Venoms
Young Adult
1107 Immunology
Publication Status: Published
Appears in Collections:National Heart and Lung Institute
Department of Medicine
Faculty of Medicine



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