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Replication of LC-MS untargeted lipidomics results in patients with calcific coronary disease: an interlaboratory reproducibility study

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Title: Replication of LC-MS untargeted lipidomics results in patients with calcific coronary disease: an interlaboratory reproducibility study
Authors: Djekic, D
Pinto, R
Vorkas, PA
Henein, MY
Item Type: Journal Article
Abstract: Background Recently a lipidomics approach was able to identify perturbed fatty acyl chain (FAC) and sphingolipid moieties that could stratify patients according to the severity of coronary calcification, a form of subclinical atherosclerosis. Nevertheless, these findings have not yet been reproduced before generalising their application. The aim of this study was to evaluate the reproducibility of lipidomics approaches by replicating previous lipidomic findings in groups of patients with calcific coronary artery disease (CCAD). Methods Patients were separated into the following groups based on their calcium score (CS); no calcification (CS: 0; n = 26), mild calcification (CS: 1–250; n = 27) and severe calcification (CS: > 250; n = 17). Two serum samples were collected from each patient and used for comparative analyses by 2 different laboratories, in different countries and time points using liquid chromatography coupled to mass spectrometry untargeted lipidomics methods. Results Six identical metabolites differentiated patients with severe coronary artery calcification from those with no calcification were found by both laboratories independently. Additionally, relative intensities from the two analyses demonstrated high correlation coefficients. Phosphatidylcholine moieties with 18-carbon FAC were identified in lower intensities and 20:4 FAC in higher intensities in the serum of diseased group. Moreover, 3 common sphingomyelins were detected. Conclusion This is the first interlaboratory reproducibility study utilising lipidomics applications in general and specifically in patients with CCAD. Lipid profiling applications in patients with CCAD are very reproducible in highly specialised and experienced laboratories and could be applied in clinical practice in order to spare patients diagnostic radiation.
Issue Date: 2-Aug-2016
Date of Acceptance: 28-Jul-2016
URI: http://hdl.handle.net/10044/1/43401
DOI: http://dx.doi.org/10.1016/j.ijcard.2016.07.214
ISSN: 1874-1754
Publisher: Elsevier
Start Page: 1042
End Page: 1048
Journal / Book Title: International Journal of Cardiology
Volume: 222
Copyright Statement: © 2016, Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords: Science & Technology
Life Sciences & Biomedicine
Cardiac & Cardiovascular Systems
Cardiovascular System & Cardiology
Lipidomics
Reproducibility
Coronary artery disease
Calcific coronary disease
Metabolomics
ARTERY CALCIFICATION
SUBCLINICAL ATHEROSCLEROSIS
CARDIOVASCULAR-DISEASE
INSULIN-RESISTANCE
PROTEOMIC APPROACH
MASS-SPECTROMETRY
BLOOD-PLASMA
FINNISH MEN
DATA SETS
UPLC-MS
Cardiovascular System & Hematology
1102 Cardiovascular Medicine And Haematology
Publication Status: Published
Appears in Collections:Division of Surgery
Faculty of Medicine



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