Soluble major histocompatibility complex class I-related chain B molecules are increased and correlate with clinical outcomes during rhinovirus infection in healthy subjects

File Description SizeFormat 
1-s2.0-S0012369215487458-main.pdfPublished version767.11 kBAdobe PDFView/Open
Title: Soluble major histocompatibility complex class I-related chain B molecules are increased and correlate with clinical outcomes during rhinovirus infection in healthy subjects
Authors: Telcian, AG
Zdrenghea, MT
Caramori, G
Laza-Stanca, V
Message, SD
Kebadze, T
Kon, OM
Groh, V
Papi, A
Johnston, SL
Mallia, P
Stanciu, LA
Item Type: Journal Article
Abstract: BACKGROUND: Surface major histocompatibility complex class I-related chain (MIC) A and B molecules are increased by IL-15 and have a role in the activation of natural killer group 2 member D-positive natural killer and CD8 T cells. MICA and MICB also exist in soluble forms (sMICA and sMICB). Rhinoviruses (RVs) are the major cause of asthma exacerbations, and IL-15 levels are decreased in the airways of subjects with asthma. The role of MIC molecules in immune responses in the lung has not been studied. Here, we determine the relationship between MICA and MICB and RV infection in vitro in respiratory epithelial cells and in vivo in healthy subjects and subjects with asthma. METHODS: Surface MICA and MICB, as well as sMICA and sMICB, in respiratory epithelial cells were measured in vitro in response to RV infection and exposure to IL-15. Levels of sMICA and sMICB in serum, sputum, and BAL were measured and correlated with blood and bronchoalveolar immune cells in healthy subjects and subjects with asthma before and during RV infection. RESULTS: RV increased MICA and MICB in vitro in epithelial cells. Exogenous IL-15 upregulated sMICB levels in RV-infected epithelial cells. Levels of sMICB molecules in serum were increased in healthy subjects compared with subjects with stable asthma. Following RV infection, airway levels of sMIC are upregulated, and there are positive correlations between sputum MICB levels and the percentage of bronchoalveolar natural killer cells in healthy subjects but not subjects with asthma. CONCLUSIONS: RV infection induces MIC molecules in respiratory epithelial cells in vitro and in vivo. Induction of MICB molecules is impaired in subjects with asthma, suggesting these molecules may have a role in the antiviral immune response to RV infections.
Issue Date: 30-Dec-2015
Date of Acceptance: 14-Jan-2014
URI: http://hdl.handle.net/10044/1/43369
DOI: https://dx.doi.org/10.1378/chest.13-2247
ISSN: 1931-3543
Publisher: Elsevier
Start Page: 32
End Page: 40
Journal / Book Title: Chest
Volume: 146
Issue: 1
Copyright Statement: © 2014 The American College of Chest Physicians. Published by Elsevier Inc. This is a Wellcome-Trust-compliant open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons. org/licenses/by/3.0/).
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Funder's Grant Number: G0601236
G1000758
Keywords: Science & Technology
Life Sciences & Biomedicine
Critical Care Medicine
Respiratory System
General & Internal Medicine
CRITICAL CARE MEDICINE
RESPIRATORY SYSTEM
RESPIRATORY EPITHELIAL-CELLS
NKG2D LIGAND EXPRESSION
C VIRUS-INFECTION
T-CELLS
NK CELLS
ACTIVATION
RECEPTOR
IL-15
ACID
CYTOKINE
Adult
Asthma
Bronchoalveolar Lavage Fluid
Cells, Cultured
Female
HLA-B27 Antigen
Humans
Immunity, Cellular
Interleukin-15
Male
Picornaviridae Infections
Reference Values
Respiratory Mucosa
T-Lymphocytes
Respiratory Mucosa
T-Lymphocytes
Cells, Cultured
Bronchoalveolar Lavage Fluid
Humans
Picornaviridae Infections
Asthma
Interleukin-15
HLA-B27 Antigen
Immunity, Cellular
Reference Values
Adult
Female
Male
Respiratory System
1103 Clinical Sciences
Publication Status: Published
Appears in Collections:National Heart and Lung Institute
Faculty of Medicine



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commonsx