Inflammation of peripheral tissues and injury to peripheral nerves induce diferring effects in the expression of the calcium-sensitive anandamide synthesising enzyme and related molecules in rat primary sensory neuron

Title: Inflammation of peripheral tissues and injury to peripheral nerves induce diferring effects in the expression of the calcium-sensitive anandamide synthesising enzyme and related molecules in rat primary sensory neuron
Author(s): Nagy, I
Suosa-Valente, J
Varga, A
Torres Perez, J
Jenes, A
Wahba, J
Mackie, K
Cravatt, B
Ueda, N
Tsuboi, K
Santha, P
Jancso, G
Tailor, H
Avelino, A
Item Type: Journal Article
Abstract: Elevation of intracellular Ca 2+ concentration induces the synthesis of N0 arachydonoylethanolamine (anandamide) in a sub0popu lation of primary sensory neurons. N0acylphosphatidylethanolamine phospholipa se D (NAPE0PLD) is the only known enzyme, which synthesises anandamide in a Ca 2+ 0dependent manner. NAPE0 PLD mRNA, as well as anandamide's main targets, the excitatory transient receptor potential vanilloid type 1 ion channel (TRPV1) and the inhibitory cannabinoid type 1 (CB1) receptor and the main anandamide0hydrolysing enzyme fatty acid amide hydrolase (FAAH) are all expressed by sub0populatio ns of nociceptive primary sensory neurons. Thus, NAPE0PLD, TRPV1, the CB1 rec eptor and FAAH could form an autocrine signalling system, which could shape t he activity of a major sub0 population of nociceptive primary sensory neurons, hence contribute to the development of pain. While the expression patterns of TRPV1, the CB1 receptor and FAAH have been comprehensively elucidated, little i s known about NAPE0PLD expression in primary sensory neurons under physiol ogical and pathological conditions. We report that NAPE0PLD is expressed by about a third of primary sensory neurons, the overwhelming majority of which also express nociceptive markers as well as the CB1 receptor, TRPV1 and FAAH . Inflammation of peripheral tissues and injury to peripheral nerves induce diff ering but concerted changes in the expression pattern of NAPE0PLD, the CB1 receptor, T RPV1 and FAAH. Together these data indicate the existence of the anatomical basis for an autocrine signalling system, in a major proportion of nociceptive primar y sensory neurons, and that alterations in that autocrine signalling by periphe ral pathologies could contribute to the development of both inflammatory and neuropathi c pain.
Publication Date: 14-Mar-2017
Date of Acceptance: 30-Nov-2016
URI: http://hdl.handle.net/10044/1/43081
DOI: https://dx.doi.org/10.1002/cne.24154
ISSN: 1096-9861
Publisher: Wiley
Start Page: 1778
End Page: 1796
Journal / Book Title: Journal of Comparative Neurology
Volume: 525
Issue: 8
Sponsor/Funder: Wellcome Trust
British Journal of Anaesthesia
Commission of the European Communities
Funder's Grant Number: 081637/Z/06/Z
DSAN_P20272
254661
Copyright Statement: © 2017 Wiley Periodicals, Inc. This is the accepted version of the following article, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/cne.24154/abstract
Keywords: Science & Technology
Life Sciences & Biomedicine
Neurosciences
Zoology
Neurosciences & Neurology
cannabinoid type 1 receptor
fatty acid amide hydrolase
inflammation
neuropathy
pain
transient receptor potential vanilloid type 1 ion channel
DORSAL-ROOT GANGLION
ACID AMIDE HYDROLASE
HYDROLYZING PHOSPHOLIPASE-D
COMPLETE FREUNDS-ADJUVANT
CANNABINOID RECEPTOR
CAPSAICIN RECEPTOR
NEUROPATHIC-PAIN
ION-CHANNEL
ACYL ETHANOLAMINE
TRPV1 FUNCTION
cannabinoid type 1 receptor
fatty acid amide hydrolase
inflammation
neuropathy
pain
transient receptor potential vanilloid type 1 ion channel
Neurology & Neurosurgery
1109 Neurosciences
0608 Zoology
1116 Medical Physiology
Publication Status: Published
Open Access location: http://onlinelibrary.wiley.com/doi/10.1002/cne.24154/epdf
Appears in Collections:Division of Surgery
Faculty of Medicine



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