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Vitamin D increases the antiviral activity of bronchial epithelial cells in vitro

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Title: Vitamin D increases the antiviral activity of bronchial epithelial cells in vitro
Authors: Telcian, AG
Zdrenghea, MT
Edwards, MR
Laza-Stanca, V
Mallia, P
Johnston, SL
Stanciu, LA
Item Type: Journal Article
Publication Date: 2017-11-10T07:00:14Z
Abstract: BACKGROUND: By modulating the antiviral immune response via vitamin D receptor, the active form of vitamin D (1,25-dihydroxyvitamin D, calcitriol) could play a central role in protection against respiratory virus infections. This in vitro study tested the hypothesis that respiratory viruses modulate vitamin D receptor expression in human bronchial epithelial cells and this modulation affects the antiviral response to exogenous vitamin D. METHODS: Human primary bronchial epithelial cells were infected with rhinoviruses and respiratory syncytial virus in the presence or absence of vitamin D. Expression of vitamin D receptor, 1α-hydroxylase (1α(OH)ase), 24-hydroxylase (24(OH)ase), innate interferons, interferon stimulated genes and cathelicidin were measured by quantitative polymerase chain reaction. The antiviral effect of vitamin D on rhinovirus replication was determined by measurement of virus load. A direct inactivation assay was used to determine the antiviral activity of cathelicidin. RESULTS: Both RV and RSV decreased vitamin D receptor and 24(OH)ase and, in addition, RSV increased 1α(OH)ase expression in epithelial cells. Vitamin D decreased rhinovirus replication and release, and increased rhinovirus-induced interferon stimulated genes and cathelicidin. Furthermore, cathelicidin had direct anti-rhinovirus activity. CONCLUSIONS: Despite lower vitamin D receptor levels in rhinovirus-infected epithelial cells, exogenous vitamin D increased antiviral defences most likely via cathelicidin and innate interferon pathways.
Issue Date: 10-Nov-2016
Date of Acceptance: 4-Nov-2016
URI: http://hdl.handle.net/10044/1/43045
DOI: http://dx.doi.org/10.1016/j.antiviral.2016.11.004
ISSN: 1872-9096
Publisher: Elsevier
Start Page: 93
End Page: 101
Journal / Book Title: Antiviral Research
Volume: 137
Copyright Statement: © 2016, Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: British Lung Foundation
Asthma UK
Funder's Grant Number: P04/13
02/027
Keywords: Cathelicidin
Interferons
Respiratory viruses
Virology
1108 Medical Microbiology
1115 Pharmacology And Pharmaceutical Sciences
Publication Status: Published
Conference Place: Netherlands
Appears in Collections:National Heart and Lung Institute
Faculty of Medicine



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