Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E

Title: Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E
Authors: Pini, C-V
Bernal, P
Godoy, P
Ramos, J-L
Segura, A
Item Type: Journal Article
Abstract: Bacterial membranes constitute the first physical barrier against different environmental stresses. Pseudomonas putida DOT-T1E accumulates cyclopropane fatty acids (CFAs) in the stationary phase of growth. In this strain the cfaB gene encodes the main cyclopropane synthase responsible of the synthesis of CFAs, and its expression is mediated by RNA polymerase with sigma factor σ38. We generated a cfaB mutant of P. putida DOT-T1E and studied its response to solvents, acid pH and other stress conditions such as temperature changes, high osmolarity and the presence of antibiotics or heavy metals in the culture medium. A CfaB knockout mutant was more sensitive to solvent stress than the wild-type strain, but in contrast to Escherichia coli and Salmonella enterica, the P. putida cfaB mutant was as tolerant to acid shock as the wild-type strain. The cfaB mutant was also as tolerant as the parental strain to a number of drugs, antibiotics and other damaging agents.
Issue Date: 18-Feb-2009
Date of Acceptance: 17-Dec-2008
URI: http://hdl.handle.net/10044/1/42640
DOI: http://dx.doi.org/10.1111/j.1751-7915.2009.00084.x
ISSN: 1751-7915
Publisher: Wiley
Start Page: 253
End Page: 261
Journal / Book Title: Microbial Biotechnology
Volume: 2
Issue: 2
Copyright Statement: © 2009 The Authors. Journal compilation © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd. Published under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/).
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Microbiology
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
Acids
Bacterial Proteins
Cyclopropanes
Fatty Acids
Methyltransferases
Molecular Sequence Data
Organic Chemicals
Pseudomonas putida
Stress, Physiological
Pseudomonas putida
Acids
Organic Chemicals
Cyclopropanes
Methyltransferases
Fatty Acids
Bacterial Proteins
Molecular Sequence Data
Stress, Physiological
0605 Microbiology
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences



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