Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor

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Title: Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor
Author(s): Dent, MR
López-Duarte, I
Dickson, CJ
Chairatana, P
Anderson, HL
Gould, IR
Wylie, D
Vyšniauskas, A
Brooks, NJ
Kuimova, MK
Item Type: Journal Article
Abstract: Molecular rotors have emerged as versatile probes of microscopic viscosity in lipid bilayers, although it has proved difficult to find probes that stain both phases equally in phase-separated bilayers. Here, we investigate the use of a membrane-targeting viscosity-sensitive fluorophore based on a thiophene moiety with equal affinity for ordered and disordered lipid domains to probe ordering and viscosity within artificial lipid bilayers and live cell plasma membranes.
Publication Date: 7-Oct-2016
Date of Acceptance: 6-Oct-2016
URI: http://hdl.handle.net/10044/1/42061
DOI: https://dx.doi.org/10.1039/c6cc05954f
ISSN: 1364-548X
Publisher: Royal Society of Chemistry
Start Page: 13269
End Page: 13272
Journal / Book Title: Chemical Communications
Volume: 52
Issue: 90
Copyright Statement: This journal is © The Royal Society of Chemistry 2016
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Imperial College Trust
Funder's Grant Number: EP/I003983/1
EP/K503381/1
EP/K030760/1
N/A
Keywords: Organic Chemistry
03 Chemical Sciences
Publication Status: Published
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Faculty of Natural Sciences



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