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Bicarbonate-induced redox tuning in Photosystem II for regulation and protection

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Title: Bicarbonate-induced redox tuning in Photosystem II for regulation and protection
Authors: Rutherford, AW
Fantuzzi, A
Brinkert, K
De Causmaecker
Kreiger-Liszkay A
Item Type: Journal Article
Abstract: The midpoint potential (Em) of QA/Q−∙AQA/QA−•, the one-electron acceptor quinone of Photosystem II (PSII), provides the thermodynamic reference for calibrating PSII bioenergetics. Uncertainty exists in the literature, with two values differing by ∼80 mV. Here, we have resolved this discrepancy by using spectroelectrochemistry on plant PSII-enriched membranes. Removal of bicarbonate (HCO3−) shifts the Em from ∼−145 mV to −70 mV. The higher values reported earlier are attributed to the loss of HCO3− during the titrations (pH 6.5, stirred under argon gassing). These findings mean that HCO3− binds less strongly when QA−• is present. Light-induced QA−• formation triggered HCO3− loss as manifest by the slowed electron transfer and the upshift in the Em of QA. HCO3−-depleted PSII also showed diminished light-induced 1O2 formation. This finding is consistent with a model in which the increase in the Em of QA/Q−∙AQA/QA−• promotes safe, direct P+∙Q−∙AP+•QA−• charge recombination at the expense of the damaging back-reaction route that involves chlorophyll triplet-mediated 1O2 formation [Johnson GN, et al. (1995) Biochim Biophys Acta 1229:202–207]. These findings provide a redox tuning mechanism, in which the interdependence of the redox state of QA and the binding by HCO3− regulates and protects PSII. The potential for a sink (CO2) to source (PSII) feedback mechanism is discussed.
Issue Date: 10-Oct-2016
Date of Acceptance: 30-Aug-2016
URI: http://hdl.handle.net/10044/1/41802
DOI: http://dx.doi.org/10.1073/pnas.1608862113
ISSN: 1091-6490
Publisher: National Academy of Sciences
Start Page: 12144
End Page: 12149
Journal / Book Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 113
Issue: 43
Copyright Statement: © 2016 National Academy of Sciences
Sponsor/Funder: The Royal Society
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: WM110001
BB/K002627/1
Keywords: CO2 fixation
photoassembly
photoinhibition
photosynthesis
water oxidation
Multidisciplinary
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences



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