Real-Time Monitoring of Ligand Binding to G-Quadruplex and Duplex DNA by Whispering Gallery Mode Sensing

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Title: Real-Time Monitoring of Ligand Binding to G-Quadruplex and Duplex DNA by Whispering Gallery Mode Sensing
Authors: Panich, S
Sleiman, MH
Steer, I
Ladame, S
Edel, JB
Item Type: Journal Article
Abstract: The therapeutic potential of small molecules targeting G-quadruplexes has gained credibility since such structures were shown to form in human cells and to be highly prevalent in the human genome, most notably at telomere ends and in oncogene promoters. Herein, we perform whispering gallery mode (WGM) sensing for monitoring DNA–small molecule interactions. Unlike most existing technologies, WGM sensing offers numerous advantages including high sensitivity, real-time analysis, easy access to kinetic parameters, and much lower cost than current gold standards. In this work, interactions of five known DNA-binding ligands with either G-quadruplex or duplex DNA immobilized on a sphere microresonator have been assessed. The induced shift of the resonant mode from quadruplex (or duplex)–ligand binding was used to estimate kinetic parameters. Association and dissociation rate constants (kon and koff, respectively) as well as dissociation equilibrium constants (KD) were measured for these five ligands binding to both duplex and quadruplex DNA.
Issue Date: 1-Sep-2016
Date of Acceptance: 1-Sep-2016
URI: http://hdl.handle.net/10044/1/41454
DOI: https://dx.doi.org/10.1021/acssensors.6b00301
ISSN: 2379-3694
Publisher: American Chemical Society
Start Page: 1097
End Page: 1102
Journal / Book Title: ACS Sensors
Volume: 1
Issue: 9
Copyright Statement: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Sensors, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/acssensors.6b00301
Publication Status: Published
Appears in Collections:Bioengineering
Chemistry
Faculty of Natural Sciences



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