Multiphoton Multispectral Fluorescence Lifetime Tomography for the Evaluation of Basal Cell Carcinomas

Title: Multiphoton Multispectral Fluorescence Lifetime Tomography for the Evaluation of Basal Cell Carcinomas
Authors: Patalay, R
Talbot, C
Alexandrov, Y
Lenz, MO
Kumar, S
Warren, S
Munro, I
Neil, MAA
Koenig, K
French, PMW
Chu, A
Stamp, GWH
Dunsby, C
Item Type: Journal Article
Abstract: We present the first detailed study using multispectral multiphoton fluorescence lifetime imaging to differentiate basal cell carcinoma cells (BCCs) from normal keratinocytes. Images were acquired from 19 freshly excised BCCs and 27 samples of normal skin (in & ex vivo). Features from fluorescence lifetime images were used to discriminate BCCs with a sensitivity/specificity of 79%/93% respectively. A mosaic of BCC fluorescence lifetime images covering >1 mm2 is also presented, demonstrating the potential for tumour margin delineation. Using 10,462 manually segmented cells from the image data, we quantify the cellular morphology and spectroscopic differences between BCCs and normal skin for the first time. Statistically significant increases were found in the fluorescence lifetimes of cells from BCCs in all spectral channels, ranging from 19.9% (425–515 nm spectral emission) to 39.8% (620–655 nm emission). A discriminant analysis based diagnostic algorithm allowed the fraction of cells classified as malignant to be calculated for each patient. This yielded a receiver operator characteristic area under the curve for the detection of BCC of 0.83. We have used both morphological and spectroscopic parameters to discriminate BCC from normal skin, and provide a comprehensive base for how this technique could be used for BCC assessment in clinical practice.
Issue Date: 11-Sep-2012
Date of Acceptance: 25-Jul-2012
URI: http://hdl.handle.net/10044/1/41327
DOI: http://dx.doi.org/10.1371/journal.pone.0043460
ISSN: 1932-6203
Publisher: Public Library of Science
Journal / Book Title: PLOS One
Volume: 7
Issue: 9
Copyright Statement: © Patalay et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/K020102/1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MULTIDISCIPLINARY SCIENCES
NONMELANOMA SKIN-CANCER
IN-VIVO
MICROSCOPY
POPULATION
DIAGNOSIS
MELANIN
TRENDS
Adolescent
Adult
Aged
Aged, 80 and over
Area Under Curve
Carcinoma, Basal Cell
Female
Humans
Male
Middle Aged
Photons
Skin Neoplasms
Spectrometry, Fluorescence
Time Factors
Tomography
Young Adult
Humans
Carcinoma, Basal Cell
Skin Neoplasms
Tomography
Spectrometry, Fluorescence
Area Under Curve
Photons
Time Factors
Adolescent
Adult
Aged
Aged, 80 and over
Middle Aged
Female
Male
Young Adult
General Science & Technology
MD Multidisciplinary
Publication Status: Published
Article Number: e43460
Appears in Collections:Physics
Photonics
Department of Medicine
Faculty of Medicine
Faculty of Natural Sciences



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