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MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - comparison with human epileptic samples

Title: MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - comparison with human epileptic samples
Authors: Roncon, P
Soukupova, M
Binaschi, A
Falcicchia, C
Zucchini, S
Ferracin, M
Langley, SR
Petretto, E
Johnson, MR
Marucci, G
Michelucci, R
Rubboli, G
Simonato, M
Item Type: Journal Article
Abstract: The identification of biomarkers of the transformation of normal to epileptic tissue would help to stratify patients at risk of epilepsy following brain injury, and inform new treatment strategies. MicroRNAs (miRNAs) are an attractive option in this direction. In this study, miRNA microarrays were performed on laser-microdissected hippocampal granule cell layer (GCL) and on plasma, at different time points in the development of pilocarpine-induced epilepsy in the rat: latency, first spontaneous seizure and chronic epileptic phase. Sixty-three miRNAs were differentially expressed in the GCL when considering all time points. Three main clusters were identified that separated the control and chronic phase groups from the latency group and from the first spontaneous seizure group. MiRNAs from rats in the chronic phase were compared to those obtained from the laser-microdissected GCL of epileptic patients, identifying several miRNAs (miR-21-5p, miR-23a-5p, miR-146a-5p and miR-181c-5p) that were up-regulated in both human and rat epileptic tissue. Analysis of plasma samples revealed different levels between control and pilocarpine-treated animals for 27 miRNAs. Two main clusters were identified that segregated controls from all other groups. Those miRNAs that are altered in plasma before the first spontaneous seizure, like miR-9a-3p, may be proposed as putative biomarkers of epileptogenesis.
Issue Date: 18-Sep-2015
Date of Acceptance: 12-Aug-2015
URI: http://hdl.handle.net/10044/1/41255
DOI: http://dx.doi.org/10.1038/srep14143
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 5
Copyright Statement: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Sponsor/Funder: Commission of the European Communities
Funder's Grant Number: 602102
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
TEMPORAL-LOBE EPILEPSY
1ST SPONTANEOUS SEIZURE
CIRCULATING MICRORNAS
LASER MICRODISSECTION
EXPRESSION PROFILE
GENE-EXPRESSION
BIOMARKERS
MODEL
PCR
MIRNAS
Adult
Animals
Biomarkers
Case-Control Studies
Cluster Analysis
Epilepsy
Female
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Hippocampus
Humans
Male
MicroRNAs
Middle Aged
Pilocarpine
Pyramidal Cells
Rats
Transcriptome
Publication Status: Published
Article Number: 14143
Appears in Collections:Clinical Sciences
Molecular Sciences
Department of Medicine
Faculty of Medicine



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