Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis

Title: Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis
Authors: Bordi, C
Lamy, M-C
Ventre, I
Termine, E
Hachani, A
Fillet, S
Roche, B
Bleves, S
Mejean, V
Lazdunski, A
Filloux, A
Item Type: Journal Article
Abstract: Bacterial pathogenesis often depends on regulatory networks, two-component systems and small RNAs (sRNAs). In Pseudomonas aeruginosa, the RetS sensor pathway downregulates expression of two sRNAs, rsmY and rsmZ. Consequently, biofilm and the Type Six Secretion System (T6SS) are repressed, whereas the Type III Secretion System (T3SS) is activated. We show that the HptB signalling pathway controls biofilm and T3SS, and fine-tunes P. aeruginosa pathogenesis. We demonstrate that RetS and HptB intersect at the GacA response regulator, which directly controls sRNAs production. Importantly, RetS controls both sRNAs, whereas HptB exclusively regulates rsmY expression. We reveal that HptB signalling is a complex regulatory cascade. This cascade involves a response regulator, with an output domain belonging to the phosphatase 2C family, and likely an anti-anti-σ factor. This reveals that the initial input in the Gac system comes from several signalling pathways, and the final output is adjusted by a differential control on rsmY and rsmZ. This is exemplified by the RetS-dependent but HptB-independent control on T6SS. We also demonstrate a redundant action of the two sRNAs on T3SS gene expression, while the impact on pel gene expression is additive. These features underpin a novel mechanism in the fine-tuned regulation of gene expression.
Issue Date: 1-Apr-2010
Date of Acceptance: 17-Mar-2010
URI: http://hdl.handle.net/10044/1/40521
DOI: http://dx.doi.org/10.1111/j.1365-2958.2010.07146.x
ISSN: 1365-2958
Publisher: Wiley
Start Page: 1427
End Page: 1443
Journal / Book Title: Molecular Microbiology
Volume: 76
Issue: 6
Copyright Statement: © 2010 Blackwell Publishing Ltd. Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://www3.interscience.wiley.com/ authorresources/onlineopen.html.
Sponsor/Funder: Medical Research Council (MRC)
Funder's Grant Number: G0800171
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Microbiology
BIOCHEMISTRY & MOLECULAR BIOLOGY
MICROBIOLOGY
MEDIATED MULTISTEP PHOSPHORELAY
VIBRIO-CHOLERAE
2-COMPONENT SYSTEMS
VIRULENCE FACTORS
BIOFILM FORMATION
III SECRETION
PAO1
GENES
IDENTIFICATION
EXPRESSION
Bacterial Proteins
Biofilms
Biological Transport
Gene Expression Regulation, Bacterial
Genes, Reporter
Models, Biological
Pseudomonas aeruginosa
RNA, Bacterial
RNA, Small Interfering
Signal Transduction
beta-Galactosidase
06 Biological Sciences
11 Medical And Health Sciences
07 Agricultural And Veterinary Sciences
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences



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