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A Quantitative Characterization of Nucleoplasmin/Histone Complexes Reveals Chaperone Versatility.

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Title: A Quantitative Characterization of Nucleoplasmin/Histone Complexes Reveals Chaperone Versatility.
Author(s): Fernández-Rivero, N
Franco, A
Velázquez-Campoy, A
Alonso, E
Muga, A
Prado, A
Item Type: Journal Article
Abstract: Nucleoplasmin (NP) is an abundant histone chaperone in vertebrate oocytes and embryos involved in storing and releasing maternal histones to establish and maintain the zygotic epigenome. NP has been considered a H2A-H2B histone chaperone, and recently it has been shown that it can also interact with H3-H4. However, its interaction with different types of histones has not been quantitatively studied so far. We show here that NP binds H2A-H2B, H3-H4 and linker histones with Kd values in the subnanomolar range, forming different complexes. Post-translational modifications of NP regulate exposure of the polyGlu tract at the disordered distal face of the protein and induce an increase in chaperone affinity for all histones. The relative affinity of NP for H2A-H2B and linker histones and the fact that they interact with the distal face of the chaperone could explain their competition for chaperone binding, a relevant process in NP-mediated sperm chromatin remodelling during fertilization. Our data show that NP binds H3-H4 tetramers in a nucleosomal conformation and dimers, transferring them to DNA to form disomes and tetrasomes. This finding might be relevant to elucidate the role of NP in chromatin disassembly and assembly during replication and transcription.
Publication Date: 25-Aug-2016
Date of Acceptance: 2-Aug-2016
URI: http://hdl.handle.net/10044/1/40084
DOI: http://dx.doi.org/10.1038/srep32114
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 6
Copyright Statement: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Publication Status: Published
Article Number: 32114
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine



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