Automated High-Throughput Identification and Characterization of Clinically Important Bacteria and Fungi using Rapid Evaporative Ionization Mass Spectrometry

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Title: Automated High-Throughput Identification and Characterization of Clinically Important Bacteria and Fungi using Rapid Evaporative Ionization Mass Spectrometry
Authors: Bolt, F
Cameron, SJ
Karancsi, T
Simon, D
Schaffer, R
Rickards, T
Hardiman, K
Burke, A
Bodai, Z
Perdones-Montero, A
Rebec, M
Balog, J
Takats, Z
Item Type: Journal Article
Abstract: Rapid Evaporative Ionisation Mass Spectrometry (REIMS) has been shown to quickly and accurately speciate microorganisms based upon their species-specific lipid profile. Previous work by members of this group showed that the use of a handheld bipolar probe allowed REIMS to analyse microbial cultures directly from culture plates, without any prior preparation. However, this method of analysis would likely be unsuitable for a high-throughput clinical microbiology laboratory. Here, we report on the creation of a customised platform which enables automated, high-throughput REIMS analysis, which requires minimal user input and operation; and suitable for use in clinical microbiology laboratories. The ability of this high-throughput platform to speciate clinically important microorganisms was tested through the analysis of 375 different clinical isolates, collected from distinct patient samples, from 25 microbial species. After optimisation of our data analysis approach, we achieved substantially similar results between the two REIMS approaches. For handheld bipolar probe REIMS a speciation accuracy of 96.3% was achieved, whilst for high-throughput REIMS, an accuracy of 93.9% was achieved. Thus, high-throughput REIMS offers an alternative mass spectrometry based method for the rapid and accurate identification of clinically important microorganisms in clinical laboratories without any pre-analysis preparative steps.
Issue Date: 25-Aug-2016
Date of Acceptance: 25-Aug-2016
ISSN: 1086-4377
Publisher: American Chemical Society
Start Page: 9419
End Page: 9426
Journal / Book Title: Analytical Chemistry
Volume: 88
Issue: 19
Copyright Statement: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © 2016 American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Micromass UK Ltd
Funder's Grant Number: BB/L020858/1
ICL 01/02/03
Keywords: Analytical Chemistry
Chemical Engineering
Other Chemical Sciences
Publication Status: Published
Appears in Collections:Division of Surgery
Faculty of Medicine

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