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Simultaneous monitoring of potassium, glucose and lactate during spreading depolarisation in the injured human brain - proof of principle of a novel real-time neurochemical analysis system, continuous online microdialysis (coMD)

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Title: Simultaneous monitoring of potassium, glucose and lactate during spreading depolarisation in the injured human brain - proof of principle of a novel real-time neurochemical analysis system, continuous online microdialysis (coMD)
Authors: Rogers, ML
Leong, CL
Gowers, SAN
Samper, IC
Jewell, SL
Khan, A
McCarthy, L
Pahl, C
Tolias, CM
Walsh, DC
Strong, AJ
Boutelle, MG
Item Type: Journal Article
Abstract: Spreading Depolarisations (SDs) occur spontaneously and frequently in injured human brain. They propagate slowly through injured tissue often cycling around a local area of damage. Tissue recovery after an SD requires greatly augmented energy utilisation to normalise ionic gradients from a virtually complete loss of membrane potential. In the injured brain this is difficult because local blood flow is often low and unreactive. In this study we use a new variant of microdialysis, continuous on-line microdialysis (coMD), to observe the effects of SDs on brain metabolism. The neurochemical changes are dynamic and take place on the timescale of the passage of an SD past the microdialysis probe. Dialysate potassium levels provide an ionic correlate of cellular depolarisation and show a clear transient increase. Dialysate glucose levels reflect a balance between local tissue glucose supply and utilization. These show a clear transient decrease of variable magnitude and duration. Dialysate lactate levels indicate non-oxidative metabolism of glucose and show a transient increase. Preliminary data suggest that the transient changes recover more slowly after the passage of a sequence of multiple SD’s giving rise to a decrease in basal dialysate glucose and an increase in basal dialysate potassium and lactate levels.
Issue Date: 1-Jan-2017
Date of Acceptance: 30-Aug-2016
URI: http://hdl.handle.net/10044/1/39579
DOI: https://dx.doi.org/10.1177/0271678X16674486
ISSN: 1559-7016
Publisher: Sage
Start Page: 1883
End Page: 1895
Journal / Book Title: Journal of Cerebral Blood Flow and Metabolism
Volume: 37
Issue: 5
Copyright Statement: © Author(s) 2016, published by Sage Publications. This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Keywords: On-line microdialysis
ischaemic brain injury
microfluidics
neurometabolic coupling
spreading depolarisation
Neurology & Neurosurgery
1103 Clinical Sciences
1109 Neurosciences
1102 Cardiovascular Medicine And Haematology
Publication Status: Published
Appears in Collections:Faculty of Engineering
Bioengineering



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