Surface Charge Measurement of SonoVue, Definity and Optison: A Comparison of Laser Doppler Electrophoresis and Micro-Electrophoresis

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Title: Surface Charge Measurement of SonoVue, Definity and Optison: A Comparison of Laser Doppler Electrophoresis and Micro-Electrophoresis
Authors: Ja'afar, F
Leow, CH
Garbin, V
Sennoga, CA
Tang, M-X
Seddon, JM
Item Type: Journal Article
Abstract: Microbubble (MB) contrast-enhanced ultrasonography is a promising tool for targeted molecular imaging. It is important to determine the MB surface charge accurately as it affects the MB interactions with cell membranes. In this article, we report the surface charge measurement of SonoVue, Definity and Optison. We compare the performance of the widely used laser Doppler electrophoresis with an in-house micro-electrophoresis system. By optically tracking MB electrophoretic velocity in a microchannel, we determined the zeta potentials of MB samples. Using micro-electrophoresis, we obtained zeta potential values for SonoVue, Definity and Optison of −28.3, −4.2 and −9.5 mV, with relative standard deviations of 5%, 48% and 8%, respectively. In comparison, laser Doppler electrophoresis gave −8.7, +0.7 and +15.8 mV with relative standard deviations of 330%, 29,000% and 130%, respectively. We found that the reliability of laser Doppler electrophoresis is compromised by MB buoyancy. Micro-electrophoresis determined zeta potential values with a 10-fold improvement in relative standard deviation.
Issue Date: 28-Aug-2015
Date of Acceptance: 6-Jul-2015
URI: http://hdl.handle.net/10044/1/39544
DOI: http://dx.doi.org/10.1016/j.ultrasmedbio.2015.07.001
ISSN: 0301-5629
Publisher: Elsevier
Start Page: 2990
End Page: 3000
Journal / Book Title: Ultrasound in Medicine and Biology
Volume: 41
Issue: 11
Copyright Statement: © 2015 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/K039946/1
Keywords: Science & Technology
Technology
Life Sciences & Biomedicine
Acoustics
Radiology, Nuclear Medicine & Medical Imaging
Microbubbles
Ultrasound contrast agents
SonoVue
Definity
Optison
Surface charge
Zeta potential
Micro-electrophoresis
Laser Doppler electrophoresis
Particle tracking
MICROBUBBLES
ULTRASOUND
AGENTS
Albumins
Contrast Media
Electrophoresis
Fluorocarbons
Phospholipids
Reproducibility of Results
Sulfur Hexafluoride
Ultrasonography, Doppler
1103 Clinical Sciences
Publication Status: Published
Appears in Collections:Faculty of Engineering
Bioengineering
Chemistry
Chemical Engineering
Faculty of Natural Sciences



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