In vitro methods for studying the mechanisms of resistance to DNA-damaging therapeutic drugs

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Title: In vitro methods for studying the mechanisms of resistance to DNA-damaging therapeutic drugs
Authors: Khongkow, P
Middleton, AK
Wong, JP
Kandola, NK
Kongsema, M
De Moraes, GN
Gomes, AR
Lam, EW
Item Type: Journal Article
Abstract: Most commonly used anticancer drugs exert their effects mainly by causing DNA damage. The enhancement in DNA damage response (DDR) is considered a key mechanism that enables cancer cells to survive through eliminating the damaged DNA lesions and thereby developing resistance to DNA-damaging agents. This chapter describes the four experimental approaches for studying DDR and genotoxic drug resistance, including the use of γ-H2AX and comet assays to monitor DNA damage and repair capacity as well as the use of clonogenic and β-galactosidase staining assays to assess long-term cell fate after DNA-damaging treatment. Finally, we also present examples of these methods currently used in our laboratory for studying the role of FOXM1 in DNA damage-induced senescence and epirubicin resistance.
Issue Date: 25-Feb-2016
Date of Acceptance: 25-Feb-2016
ISSN: 1940-6029
Publisher: Springer Verlag
Start Page: 39
End Page: 53
Journal / Book Title: Methods in Molecular Biology
Volume: 1395
Copyright Statement: The final publication is available at Springer via
Sponsor/Funder: Royal Thai Embassy
Royal Thai Embassy
Funder's Grant Number: WSCC_P37917
Keywords: Clonogenic assay
Comet assay
DNA damage
β-Galactosidase staining
Developmental Biology
0601 Biochemistry And Cell Biology
Publication Status: Published
Appears in Collections:Division of Surgery
Division of Cancer
Faculty of Medicine

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