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Development of a pipeline for exploratory metabolic profiling of infant urine

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Title: Development of a pipeline for exploratory metabolic profiling of infant urine
Authors: Jackson, FL
Georgakopoulou, N
Kaluarachchi, M
Kyriakides, M
Andreas, N
Przysiezna, N
Hyde, M
Modi, N
Nicholson, J
Wijeyesekera, A
Holmes, E
Item Type: Journal Article
Abstract: Numerous metabolic profiling pipelines have been developed to characterize the composition of human biofluids and tissues, the vast majority of these being for studies in adults. To accommodate limited sample volume and to take into account the compositional differences between adult and infant biofluids, we developed and optimized sample handling and analytical procedures for studying urine from newborns. A robust pipeline for metabolic profiling using NMR spectroscopy was established, encompassing sample collection, preparation, spectroscopic measurement and computational analysis. Longitudinal samples were collected from five infants from birth until 14 months of age. Methods of extraction, effects of freezing and sample dilution were assessed and urinary contaminants from breakdown of polymers in a range of diapers and cotton wool balls were identified and compared, including propylene glycol, acrylic acid and tert-butanol. Finally, assessment of urinary profiles obtained over the first few weeks of life revealed a dramatic change in composition, with concentrations of phenols, amino acids and betaine altering systematically over the first few months of life. Therefore, neonatal samples require more stringent standardization of experimental design, sample handling and analysis compared to adult samples in order to accommodate the variability and limited sample volume.
Issue Date: 1-Aug-2016
Date of Acceptance: 27-Jul-2016
URI: http://hdl.handle.net/10044/1/38603
DOI: https://dx.doi.org/10.1021/acs.jproteome.6b00234
ISSN: 1535-3907
Publisher: American Chemical Society
Start Page: 3432
End Page: 3440
Journal / Book Title: Journal of Proteome Research
Volume: 15
Issue: 9
Copyright Statement: This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Sponsor/Funder: Imperial College Trust
British Heart Foundation
Imperial College Healthcare NHS Trust- BRC Funding
Funder's Grant Number: PC2859ICT
PG/13/49/30307
RDB03 79560
Keywords: NMR spectroscopy
infant
metabolic profiling
metabonomics
pipeline development
urine
Biochemistry & Molecular Biology
06 Biological Sciences
03 Chemical Sciences
Publication Status: Published
Appears in Collections:Division of Surgery
Department of Medicine
Faculty of Medicine



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