A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A

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Title: A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A
Author(s): Truelove, S
Zhu, H
Lessler, J
Riley, S
Read, JM
Wang, S
Kwok, KO
Guan, Y
Jiang, CQ
Cummings, DA
Item Type: Journal Article
Abstract: BACKGROUND: Serum antibody to influenza can be used to identify past exposure and measure current immune status. The two most common methods for measuring this are the hemagglutination inhibition assay (HI) and the viral neutralization assay (NT), which have not been systematically compared for a large number of influenza viruses. METHODS: 151 study participants from near Guangzhou, China were enrolled in 2009 and provided serum. HI and NT assays were performed for 12 historic and recently circulating strains of seasonal influenza A. We compared titers using Spearman correlation and fit models to predict NT using HI results. RESULTS: We observed high positive mean correlation between HI and NT assays (Spearman's rank correlation, rho=0.86) across all strains. Correlation was highest within subtypes and within close proximity in time. Overall, an HI=20 corresponded to NT=10, and HI=40 corresponded to NT=20. Linear regression of log(NT) on log(HI) was statistically significant, with age modifying this relationship. Strain-specific area under a curve (AUC) indicated good accuracy (>80%) for predicting NT with HI. CONCLUSIONS: While we found high overall correspondence of titers between NT and HI assays for seasonal influenza A, no exact equivalence between assays could be determined. This was further complicated by correspondence between titers changing with age. These findings support generalized comparison of results between assays and give further support for use of the hemagglutination inhibition assay over the more resource intensive viral neutralization assay for seasonal influenza A, though attention should be given to the effect of age on these assays. This article is protected by copyright. All rights reserved.
Publication Date: 27-Aug-2016
Date of Acceptance: 29-Jun-2016
URI: http://hdl.handle.net/10044/1/37571
DOI: https://dx.doi.org/10.1111/irv.12408
ISSN: 1750-2640
Publisher: Wiley
Start Page: 518
End Page: 524
Journal / Book Title: Influenza Other Respiratory Viruses
Volume: 10
Issue: 6
Sponsor/Funder: Wellcome Trust
National Institutes of Health
Medical Research Council (MRC)
National Institute for Health Research
National Institute for Health Research
National Institutes of Health
Burroughs Wellcome Fund
Wellcome Trust
Wellcome Trust
Funder's Grant Number: 093488/Z/10/Z
Copyright Statement: Creative Commons Attribution 4.0 International (CC BY 4.0)
Keywords: cross-protection
hemagglutination inhibition test
microneutralization test
neutralization test
1103 Clinical Sciences
1117 Public Health And Health Services
Publication Status: Published
Open Access location: http://onlinelibrary.wiley.com/doi/10.1111/irv.12408/abstract?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+30th+July+2016+from+08:00-11:00+BST+/+03:00-06:00+EST+/+15:00-18:00+SGT+for+essential+maintenance.Apologies+for+the+inconvenience.
Appears in Collections:Faculty of Medicine
Epidemiology, Public Health and Primary Care

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