Recognizing and engineering digital-like logic gates and switches in gene regulatory networks

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Title: Recognizing and engineering digital-like logic gates and switches in gene regulatory networks
Author(s): Bradley, RW
Buck, M
Wang, B
Item Type: Journal Article
Abstract: A central aim of synthetic biology is to build organisms that can perform useful activities in response to specified conditions. The digital computing paradigm which has proved so successful in electrical engineering is being mapped to synthetic biological systems to allow them to make such decisions. However, stochastic molecular processes have graded input-output functions, thus, bioengineers must select those with desirable characteristics and refine their transfer functions to build logic gates with digital-like switching behaviour. Recent efforts in genome mining and the development of programmable RNA-based switches, especially CRISPRi, have greatly increased the number of parts available to synthetic biologists. Improvements to the digital characteristics of these parts are required to enable robust predictable design of deeply layered logic circuits.
Publication Date: 19-Jul-2016
Date of Acceptance: 6-Jul-2016
URI: http://hdl.handle.net/10044/1/37396
DOI: https://dx.doi.org/10.1016/j.mib.2016.07.004
ISSN: 1879-0364
Publisher: Elsevier
Start Page: 74
End Page: 82
Journal / Book Title: Current Opinion in Microbiology
Volume: 33
Copyright Statement: © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/K016288/1
Keywords: 0605 Microbiology
1108 Medical Microbiology
Publication Status: Published
Appears in Collections:Cell and Molecular Biology
Faculty of Natural Sciences



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